Active Recombinant Full Length Human GRK5 Protein, N-His-tagged

• Cat.No.: GRK5-75HFL
• Product Overview: N-terminal 6His-tagged, recombinant, full length, human GRK5 expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTA agarose

Specification

• Description: GRK5 is a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates the activated forms of G protein-coupled receptors thus initiating their deactivation. It has also been shown to play a role in regulating the motility of polymorphonuclear leukocytes (PMNs).
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: Full Length
• Form: 0.985mg/ml of enzyme in 50mM Tris/HCl pH8.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 204U/mg, where one unit of GRK5, active activity is defined as 1nmol phosphate incorporated into 2mg/ml casein per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 71.6 kDa
• AA Sequence: 1 MSYYHHHHHH DYDIPTTENL YFQGAMDPEF MELENIVANT VLLKAREGGG GKRKGKSKKW 61 KEILKFPHIS QCEDLRRTID RDYCSLCDKQ PIGRLLFRQF CETRPGLECY IQFLDSVAEY 121 EVTPDEKLGE KGKEIMTKYL TPKSPVFIAQ VGQDLVSQTE EKLLQKPCKE LFSACAQSVH 181 EYLRGEPFHE YLDSMFFDRF LQWKWLERQP VTKNTFRQYR VLGKGGFGEV CACQVRATGK 241 MYACKRLEKK RIKKRKGESM ALNEKQILEK VNSQFVVNLA YAYETKDALC LVLTIMNGGD 301 LKFHIYNMGN PGFEEERALF YAAEILCGLE DLHRENTVYR DLKPENILLD DYGHIRISDL 361 GLAVKIPEGD LIRGRVGTVG YMAPEVLNNQ RYGLSPDYWG LGCLIYEMIE GQSPFRGRKE 421 KVKREEVDRR VLETEEVYSH KFSEEAKSIC KMLLTKDAKQ RLGCQEEGAA EVKRHPFFRN 481 MNFKRLEAGM LDPPFVPDPR AVYCKDVLDI EQFSTVKGVN LDHTDDDFYS KFSTGSVSIP 541 WQNEMIETEC FKELNVFGPN GTLPPDLNRN HPPEPPKKGL LQRLFKRQHQ NNSKSSPSSK 601 TSFNHHINSN HVSSNSTGSS
• Purity: Purity 96.6% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: GRK5 G protein-coupled receptor kinase 5 [ Homo sapiens (human) ]
• Official Symbol: GRK5
• Synonyms: GRK5; G protein-coupled receptor kinase 5; GPRK5; FP2025
• Gene ID: 2869
• mRNA Refseq: NM_005308.3
• Protein Refseq: NP_005299.1
• MIM: 600870
• UniProt ID: P34947