Recombinant Human GRK5 cell lysate

• Cat.No.: GRK5-640HCL
• Product Overview: Human GRK5 / GPRK5 derived in Baculovirus-Insect cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls

Specification

• Source: Baculovirus-Insect Cells
• Species: Human
• Preparation method: Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
• Lysis buffer: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Quality control Testing: 12.5% SDS-PAGE Stained with Coomassie Blue
• Recommended Usage: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
• Stability: Samples are stable for up to twelve months from date of receipt at -80°C
• Storage Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Storage Instruction: Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.

Gene Information

• Gene Name: GRK5 G protein-coupled receptor kinase 5 [ Homo sapiens ]
• Official Symbol: GRK5
• Synonyms: GRK5; G protein-coupled receptor kinase 5; GPRK5; FP2025; g protein-coupled receptor kinase GRK5; FLJ39780
• Gene ID: 2869
• mRNA Refseq: NM_005308
• Protein Refseq: NP_005299
• MIM: 600870
• UniProt ID: P34947
• Chromosome Location: 10q24-qter
• Pathway: Alpha-synuclein signaling, organism-specific biosystem; Calcium Regulation in the Cardiac Cell, organism-specific biosystem; Chemokine signaling pathway, organism-specific biosystem; Chemokine signaling pathway, conserved biosystem; Endocytosis, organism-specific biosystem; Endocytosis, conserved biosystem; G alpha (q) signalling events, organism-specific biosystem
• Function: ATP binding; G-protein coupled receptor kinase activity; nucleotide binding; phospholipid binding; protein binding; protein kinase C binding; protein serine/threonine kinase activity

Reviews

09/06/2021

Good product, fast delivery.

05/08/2021

Highly recommend, very satisfied.

03/05/2021

Worked perfectly, great quality.

Q&As

What therapeutic strategies are being explored involving GRK5 modulation?

Strategies include developing GRK5 inhibitors for potential use in heart disease and Alzheimer’s.

Are there known genetic mutations in GRK5 associated with specific health conditions?

Certain GRK5 polymorphisms are associated with hypertension and heart failure risk.

How is GRK5 implicated in neurodegenerative diseases?

GRK5 has been linked to Alzheimer’s disease through its role in tau phosphorylation.

How does GRK5 regulate G protein-coupled receptor (GPCR) activity?

It phosphorylates activated GPCRs, promoting β-arrestin binding and receptor internalization.

In which tissues or cell types is GRK5 predominantly expressed?

GRK5 is widely expressed but has notable levels in the heart and brain.

What role does GRK5 play in cardiovascular function?

It regulates cardiac contractility and blood pressure, influencing heart function.

What is the main function of GRK5 in cellular signaling?

GRK5 phosphorylates and desensitizes GPCRs, modulating their signaling.