Active Recombinant Full Length Human GRK7 Protein, N-His-tagged

• Cat.No.: GRK7-77HFL
• Product Overview: N-terminal 6His-tagged, recombinant, full length human GRK7 expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTA agarose.

Specification

• Description: GRK7 (G Protein-Coupled Receptor Kinase 7) is a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. It is specifically expressed in the retina and the encoded protein has been shown to phosphorylate cone opsins and initiate their deactivation. It involved in the shutoff of the photoresponse and adaptation to changing light conditions via cone opsin phosphorylation, including rhodopsin (RHO).
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: Full Length
• Form: 1.91mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 301U/mg, where one unit of GRK7, active activity is defined as 1nmol phosphate incorporated into 2mg/ml casein per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 66 kDa
• AA Sequence: 1 MSYYHHHHHH DYDIPTTENL YFQGAMDPEF MVDMGALDNL IANTAYLQAR KPSDCDSKEL 61 QRRRRSLALP GLQGCAELRQ KLSLNFHSLC EQQPIGRRLF RDFLATVPTF RKAATFLEDV 121 QNWELAEEGP TKDSALQGLV ATCASAPAPG NPQPFLSQAV ATKCQAATTE EERVAAVTLA 181 KAEAMAFLQE QPFKDFVTSA FYDKFLQWKL FEMQPVSDKY FTEFRVLGKG GFGEVCAVQV 241 KNTGKMYACK KLDKKRLKKK GGEKMALLEK EILEKVSSPF IVSLAYAFES KTHLCLVMSL 301 MNGGDLKFHI YNVGTRGLDM SRVIFYSAQI ACGMLHLHEL GIVYRDMKPE NVLLDDLGNC 361 RLSDLGLAVE MKGGKPITQR AGTNGYMAPE ILMEKVSYSY PVDWFAMGCS IYEMVAGRTP 421 FKDYKEKVSK EDLKQRTLQD EVKFQHDNFT EEAKDICRLF LAKKPEQRLG SREKSDDPRK 481 HHFFKTINFP RLEAGLIEPP FVPDPSVVYA KDIAEIDDFS EVRGVEFDDK DKQFFKNFAT 541 GAVPIAWQEE IIETGLFEEL NDPNRPTGCE EGNSSKSGVC LLL
• Purity: Purity 99% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: GRK7 G protein-coupled receptor kinase 7 [ Homo sapiens (human) ]
• Official Symbol: GRK7
• Synonyms: GRK7; G protein-coupled receptor kinase 7; GPRK7
• Gene ID: 131890
• mRNA Refseq: NM_139209.3
• Protein Refseq: NP_631948.1
• MIM: 606987
• UniProt ID: Q8WTQ7