Recombinant Human G Protein-Coupled Receptor Kinase 7, GST-tagged

• Cat.No.: GRK7-1021H
• Product Overview: Full-length recombinant human GRK7 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: GST
• Description: GRK7 is a member of the G protein-coupled receptor kinase subfamily and is a Ser/Thr protein kinase. GRK7 is specifically expressed in the retina and has been shown to phosphorylate cone opsins and initiate their deactivation. GRK7 catalyzes rhodopsin phosphorylation in a light-dependent manner. GRK7 co-localizes with GRK1 in human cone outer segments.
• Applications: Kinase Assay; Western Blot
• Molecular Weight: 89 kDa
• Expression System: Sf9 insect cells using baculovirus
• Form: Recombinant protein stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.
• Purity: >95%
• Concentration: 0.1 ug/ul
• Specific Activity: 2.5 nmol/min/mg
• Sequences: Full length
• Storage: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Pathways: Chemokine signaling pathway; Endocytosis; Phototransduction; Visual signal transduction: Cones

Gene Information

• Gene Name: GRK7 G protein-coupled receptor kinase 7 [ Homo sapiens ]
• Official Symbol: GRK7
• Synonyms: GRK7; G protein-coupled receptor kinase 7; GPRK7; G protein-coupled receptor kinase 7; OTTHUMP00000214002; g protein-coupled receptor kinase GRK7
• Gene ID: 131890
• mRNA Refseq: NM_139209
• Protein Refseq: NP_631948
• MIM: 606987
• UniProt ID: Q8WTQ7
• Chromosome Location: 3q24
• Function: ATP binding; G-protein coupled receptor kinase activity; nucleotide binding; rhodopsin kinase activity; signal transducer activity