Active Recombinant Full Length Human NEK11 Protein, N-His-tagged

• Cat.No.: NEK11-114HFL
• Product Overview: N-terminal 6Histagged, recombinant, human full length, NEK11, expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTA agarose.

Specification

• Description: NEK11 is a protein kinase which plays an important role in the G2/M checkpoint response to DNA damage. Controls degradation of CDC25A by directly phosphorylating it on residues whose phosphorylation is required for BTRC-mediated polyubiquitination and degradation.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: Full Length
• Form: 1.72mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 275U/mg, where one unit of NEK11, active activity is defined as 1nmol phosphate incorporated into 0.33mg/ml myelin basic protein (MBP) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 59.3 kDa
• AA Sequence: 1 MSYYHHHHHH DYDIPTTENL YFQGAMDPEF MLKFQEAAKC VSGSTAISTY PKTLIARRYV 61 LQQKLGSGSF GTVYLVSDKK AKRGEELKVL KEISVGELNP NETVQANLEA QLLSKLDHPA 121 IVKFHASFVE QDNFCIITEY CEGRDLDDKI QEYKQAGKIF PENQIIEWFI QLLLGVDYMH 181 ERRILHRDLK SKNVFLKNNL LKIGDFGVSR LLMGSCDLAT TLTGTPHYMS PEALKHQGYD 241 TKSDIWSLAC ILYEMCCMNH AFAGSNFLSI VLKIVEGDTP SLPERYPKEL NAIMESMLNK 301 NPSLRPSAIE ILKIPYLDEQ LQNLMCRYSE MTLEDKNLDC QKEAAHIINA MQKRIHLQTL 361 RALSEVQKMT PRERMRLRKL QAADEKARKL KKIVEEKYEE NSKRMQELRS RNFQQLSVDV 421 LHEKTHLKGM EEKEEQPEGR LSCSPQDEDE ERWQGREEES DEPTLENLPE SQPIPSMDLH 481 ELESIVEDAT SDLGYHGDCN LISLDEYWKN EK
• Purity: Purity 77% by SDSPAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: NEK11 NIMA related kinase 11 [ Homo sapiens (human) ]
• Official Symbol: NEK11
• Synonyms: NEK11; NIMA related kinase 11; FLJ23495
• Gene ID: 79858
• mRNA Refseq: NM_001146003.2
• Protein Refseq: NP_001139475.1
• MIM: 609779
• UniProt ID: Q8NG66