Active Recombinant Full Length Human PIP5K1C Protein, N-His-tagged

• Cat.No.: PIP5K1C-148HFL
• Product Overview: N-terminal 6Histagged, recombinant human PIP5K1γ full length. Expressed by baculovirus in Sf21 insect cells. Purified using immobilized metal affinity chromatography.

Specification

• Description: PIP5K1γ protein is a member of the type I phosphatidylinositol-4-phosphate 5-kinase family of enzymes. A similar protein in mice is found in synapses and focal adhesion plaques, and binds the FERM domain of talin through its C-terminus.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: Full Length
• Form: 2.28mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 0.2mM PMSF, 1mM benzamidine, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 2046U/mg, where one unit of PIP5K1γ, active activity is defined as 1nmol phosphatidylinositol 4,5-bisphosphate (PIP2) formed per minute at room temperature with a final ATP concentration of 200μM.
• Molecular Mass: 77 kDa
• AA Sequence: 1 MSYYHHHHHH DYDIPTTENL YFQGAMDPEF MELEVPDEAE SAEAGAVPSE AAWAAESGAA 61 AGLAQKKAAP TEVLSMTAQP GPGHGKKLGH RGVDASGETT YKKTTSSTLK GAIQLGIGYT 121 VGHLSSKPER DVLMQDFYVV ESIFFPSEGS NLTPAHHFQD FRFKTYAPVA FRYFRELFGI 181 RPDDYLYSLC NEPLIELSNP GASGSLFYVT SDDEFIIKTV MHKEAEFLQK LLPGYYMNLN 241 QNPRTLLPKF YGLYCVQSGG KNIRVVVMNN ILPRVVKMHL KFDLKGSTYK RRASKKEKEK 301 SFPTYKDLDF MQDMPEGLLL DADTFSALVK TLQRDCLVLE SFKIMDYSLL LGVHNIDQHE 361 RERQAQGAQS TSDEKRPVGQ KALYSTAMES IQGGAARGEA IESDDTMGGI PAVNGRGERL 421 LLHIGIIDIL QSYRFIKKLE HTWKALVHDG DTVSVHRPSF YAERFFKFMS NTVFRKNSSL 481 KSSPSKKGRG GALLAVKPLG PTAAFSASQI PSEREEAQYD LRGARSYPTL EDEGRPDLLP 541 CTPPSFEEAT TASIATTLSS TSLSIPERSP SETSEQPRYR RRTQSSGQDG RPQEEPPAEE 601 DLQQITVQVE PACSVEIVVP KEEDAGVEAS PAGASAAVEV ETASQASDEE GAPASQASDE 661 EDAPATDIYF PTDERSWVYS PLHYSAQAPP ASDGESDT
• Purity: Purity 87% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: PIP5K1C phosphatidylinositol-4-phosphate 5-kinase type 1 gamma [ Homo sapiens (human) ]
• Official Symbol: PIP5K1C
• Synonyms: PIP5K1C; phosphatidylinositol-4-phosphate 5-kinase type 1 gamma; LCCS3; PIP5Kgamma; PIP5K-GAMMA; PIP5K1-gamma
• Gene ID: 23396
• mRNA Refseq: NM_012398.3
• Protein Refseq: NP_036530.1
• MIM: 606102
• UniProt ID: O60331