Active Recombinant Full Length Human PRKDC Protein, Flag-tagged

• Cat.No.: PRKDC-64HFL
• Product Overview: Recombinant, human FLAG-tagged DNA-PK (GenBank NM_006904.6) full length, expressed in a mammalian cell line.

Specification

• Description: DNA-PK is a serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. Must be bound to DNA to express its catalytic properties. Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C). Recruited by XRCC5 and XRCC6 to DNA ends and is required to protect and align broken ends of DNA, thereby preventing their degradation, and sequester the DSB for repair by NHEJ. Act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step. Found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion (By similarity). Also involved in modulation of transcription.
• Source: Mammalian cell line.
• Species: Human
• Tag: Flag
• Protein length: Full Length
• Form: 0.057mg/ml of enzyme in storage buffer.
• Bio-activity: This lot of DNA-PK is active when tested using c-Myc, GST-tagged, recombinant human full length p53 produced in E coli as the substrate, and meets product specifications.
• Molecular Mass: 470 kDa
• Purity: Purity 67% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 1 year at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -20 centigrade. Unopened reagent is stable for a minimum of 6 months from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: PRKDC protein kinase, DNA-activated, catalytic subunit [ Homo sapiens (human) ]
• Official Symbol: PRKDC
• Synonyms: PRKDC; protein kinase, DNA-activated, catalytic subunit; HYRC; p350; DNAPK; DNPK1; HYRC1; IMD26; XRCC7; DNAPKc; DNA-PKC; DNA-PKcs
• Gene ID: 5591
• mRNA Refseq: NM_006904.7
• Protein Refseq: NP_008835.5
• MIM: 600899
• UniProt ID: P78527