Recombinant Human PRKDC Protein

• Cat.No.: PRKDC-860H
• Product Overview: Recombinant Human PRKDC Protein was expressed in HeLa cells.

Specification

• Description: DNA-Dependent Protein Kinase (DNA-PK) phosphorylates several DNA-binding substrates in vitro, including the tumor suppressor protein p53, the SV40 large T antigen and several transcription factors. DNA-PK is thought to play a role in controlling gene regulation and cell growth.
• Source: HeLa cells
• Species: Human
• Form: 25mM HEPES (pH 7.5), 50mM KCl, 0.2mM EDTA, 10mM MgCl2, 1mM DTT, 10% glycerol.
• Molecular Mass: DNA-Dependent Protein Kinase (DNA-PK) consists of an approximate 460kDa catalytic subunit and a heterodimeric DNA-binding subunit (Ku) containing a 85kDa and a 70kDa peptide (1). It is purified from HeLa cells.
• Unit Definition: One unit is defined as the amount of enzyme required to transfer 1pmol of phosphate to DNA-Dependent Protein Kinase Peptide Substrate in 1 minute at 30 centigrade.
• Storage: DNA-PK is stable at 4 centigrade for 1 hour. Avoid multiple freeze-thaw cycles or exposure to frequent temperature changes. These fluctuations can greatly alter product stability.
• Publication:
  DNA-PKcs as an upstream mediator of OCT4-induced MYC activation in small cell lung cancer (2023)

Gene Information

• Gene Name: PRKDC protein kinase, DNA-activated, catalytic polypeptide [ Homo sapiens ]
• Official Symbol: PRKDC
• Synonyms: PRKDC; protein kinase, DNA-activated, catalytic polypeptide; HYRC, HYRC1; DNA-dependent protein kinase catalytic subunit; DNA PKcs; DNAPK; DNPK1; p350; XRCC7; p460; DNA-PK catalytic subunit; hyper-radiosensitivity of murine scid mutation, complementing 1; HYRC; HYRC1; DNA-PKcs
• Gene ID: 5591
• mRNA Refseq: NM_001081640
• Protein Refseq: NP_001075109
• MIM: 600899
• UniProt ID: P78527