Active Recombinant Full Length Human PTK6 Protein, N-His-tagged

• Cat.No.: PTK6-28HFL
• Product Overview: N-terminal, 6Histagged, recombinant, full-length, human BRK, expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTAagarose.

Specification

• Description: BRK (breast tumor kinase, PTK6) is a non-receptor tyrosine kinase containing SH3, SH2, and tyrosine kinase catalytic domains. BRK overexpression is frequently observed in breast cancer and several other cancer types. Studies reveal BRK translocates to membrane ruffles, where it colocalizes with paxillin during cell migration. Inhibition of BRK may be useful for the treatment of breast and other cancers.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: Full Length
• Form: 0.82mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.03% Brij-35, 0.1mM EGTA, 270mM sucrose, 0.2mM PMSF, 1mM benzamidine, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 145U/mg, where one unit of BRK, active activity is defined as 1nmol phosphate incorporated into 0.1mg/ml poly(Glu, Tyr) (4:1) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 55.3 kDa
• AA Sequence: 1 MSYYHHHHHH DYDIPTTENL YFQGAMGSMV SRDQAHLGPK YVGLWDFKSR TDEELSFRAG 61 DVFHVARKEE QWWWATLLDE AGGAVAQGYV PHNYLAERET VESEPWFFGC ISRSEAVRRL 121 QAEGNATGAF LIRVSEKPSA DYVLSVRDTQ AVRHYKIWRR AGGRLHLNEA VSFLSLPELV 181 NYHRAQSLSH GLRLAAPCRK HEPEPLPHWD DWERPREEFT LCRKLGSGYF GEVFEGLWKD 241 RVQVAIKVIS RDNLLHQQML QSEIQAMKKL RHKHILALYA VVSVGDPVYI ITELMAKGSL 301 LELLRDSDEK VLPVSELLDI AWQVAEGMCY LESQNYIHRD LAARNILVGE NTLCKVGDFG 361 LARLIKEDVY LSHDHNIPYK WTAPEALSRG HYSTKSDVWS FGILLHEMFS RGQVPYPGMS 421 NHEAFLRVDA GYRMPCPLEC PPSVHKLMLT CWCRDPEQRP CFKALRERLS SFTSYENPT
• Purity: Purity 77% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -20 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -20 centigrade. Unopened reagent is stable for a minimum of 6 months from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: PTK6 protein tyrosine kinase 6 [ Homo sapiens (human) ]
• Official Symbol: PTK6
• Synonyms: PTK6; protein tyrosine kinase 6; BRK
• Gene ID: 5753
• mRNA Refseq: NM_005975.4
• Protein Refseq: NP_005966.1
• MIM: 602004
• UniProt ID: Q13882