Active Recombinant Full Length Human SYK Protein, N-His-tagged

• Cat.No.: SYK-175HFL
• Product Overview: N-terminal 6Histagged recombinant full-length human Syk, expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTA-agrose.

Specification

• Description: SYK tyrosine kinase (spleen tyrosine kinase) is a key molecule in the development of the B cell lineage and the activation of B lymphocytes after Ag recognition by the B-cell receptor. Syk binds to ITAM motifs and initiates signaling pathway such as Ras, PI3 Kinase, Ca2+ flux , and PLCγ2. SYK is required for early B cell development.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: Full Length
• Form: 1.224mg/ml of enzyme in 50mM Tris/HCl pH7.0, 0.1mM EGTA, 150mM NaCl, 0.03% Brij-35, 0.2mM PMSF, 1mM benzamidine, 0.1% 2-mercaptoethanol, 270mM sucrose. Frozen solution.
• Bio-activity: 337U/mg, where one unit of Syk activity is defined as 1nmol phosphate incorporated into 0.1mg/ml poly(Glu, Tyr) (4:1) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 73 kDa
• AA Sequence: 1 MHHHHHHMAS SGMADSANHL PFFFGNITRE EAEDYLVQGG MSDGLYLLRQ SRNYLGGFAL 61 SVAHGRKAHH YTIERELNGT YAIAGGRTHA SPADLCHYHS QESDGLVCLL KKPFNRPQGV 121 QPKTGPFEDL KENLIREYVK QTWNLQGQAL EQAIISQKPQ LEKLIATTAH EKMPWFHGKI 181 SREESEQIVL IGSKTNGKFL IRARDNNGSY ALCLLHEGKV LHYRIDKDKT GKLSIPEGKK 241 FDTLWQLVEH YSYKADGLLR VLTVPCQKIG TQGNVNFGGR PQLPGSHPAT WSAGGIISRI 301 KSYSFPKPGH RKSSPAQGNR QESTVSFNPY EPELAPWAAD KGPQREALPM DTEVYESPYA 361 DPEEIRPKEV YLDRKLLTLE DKELGSGNFG TVKKGYYQMK KVVKTVAVKI LKNEANDPAL 421 KDELLAEANV MQQLDNPYIV RMIGICEAES WMLVMEMAEL GPLNKYLQQN RHVKDKNIIE 481 LVHQVSMGMK YLEESNFVHR DLAARNVLLV TQHYAKISDF GLSKALRADE NYYKAQTHGK 541 WPVKWYAPEC INYYKFSSKS DVWSFGVLMW EAFSYGQKPY RGMKGSEVTA MLEKGERMGC 601 PAGCPREMYD LMNLCWTYDV ENRPGFAAVE LRLRNYYYDV VN
• Purity: Purity 83.4% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 1 year at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: SYK spleen associated tyrosine kinase [ Homo sapiens (human) ]
• Official Symbol: SYK
• Synonyms: SYK; spleen associated tyrosine kinase; IMD82; p72-Syk
• Gene ID: 6850
• mRNA Refseq: NM_003177.7
• Protein Refseq: NP_003168.2
• MIM: 600085
• UniProt ID: P43405