Active Recombinant Full Length Mouse BLK Protein, N-His-tagged

• Cat.No.: BLK-26MFL
• Product Overview: N-terminal 6Histagged, recombinant full-length mouse Blk, expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTA agarose.

Specification

• Description: Activation of B cells by various ligands is accompanied by activation of Src-family protein tyrosine kinases. Cross-linking of the B-cell receptor leads to the activation of Src-family PTKs BLK (B-cell lymphocyte kinase), Fyn, and Lyn. Specific B and T cell progenitor subsets are preferentially susceptible to transformation by BLK and suggest a role for BLK in the control of proliferation during B cell development. BLK protein tyrosine kinase is found primarily in hematopoietic cells and inhibitors may be of therapeutic use in the treatment of B cell lymphoma.
• Source: Sf21
• Species: Mouse
• Tag: His
• Protein length: Full Length
• Form: 2.012mg/ml of 50mM Tris/HCl pH7.5, 0.1mM EGTA, 150mM NaCl, 1mM benzamidine, 0.03% Brij-35, 0.2mM PMSF, 0.1% 2-mercaptoethanol, 270mM sucrose. Frozen solution.
• Bio-activity: 294U/mg, where one unit of Blk, active activity is defined as 1nmol phosphate incorporated into 0.1mg/ml poly(Glu, Tyr) (4:1), per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 57.7 kDa
• AA Sequence: 1 MHHHHHHMGL LSSKRQVSEK GKGWSPVKIR TQDKAPPPLP PLVVFNHLAP PSPNQDPDEE 61 ERFVVALFDY AAVNDRDLQV LKGEKLQVLR STGDWWLARS LVTGREGYVP SNFVAPVETL 121 EVEKWFFRTI SRKDAERQLL APMNKAGSFL IRESESNKGA FSLSVKDITT QGEVVKHYKI 181 RSLDNGGYYI SPRITFPTLQ ALVQHYSKKG DGLCQKLTLP CVNLAPKNLW AQDEWEIPRQ 241 SLKLVRKLGS GQFGEVWMGY YKNNMKVAIK TLKEGTMSPE AFLGEANVMK TLQHERLVRL 301 YAVVTREPIY IVTEYMARGC LLDFLKTDEG SRLSLPRLID MSAQVAEGMA YIERMNSIHR 361 DLRAANILVS ETLCCKIADF GLARIIDSEY TAQEGAKFPI KWTAPEAIHF GVFTIKADVW 421 SFGVLLMEIV TYGRVPYPGM SNPEVIRSLE HGYRMPCPET CPPELYNDII TECWRGRPEE 481 RPTFEFLQSV LEDFYTATEG QYELQP
• Purity: Purity 88% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 1 year at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -20 centigrade. Unopened reagent is stable for a minimum of 6 months from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: Blk B lymphoid kinase [ Mus musculus (house mouse) ]
• Official Symbol: BLK
• Synonyms: BLK; BLK proto-oncogene, Src family tyrosine kinase; BLK; MGC10442
• Gene ID: 12143
• mRNA Refseq: NM_007549.2
• Protein Refseq: NP_031575.2
• UniProt ID: P16277