Active Recombinant Human ACVR1(147-end) Protein, N-His-tagged

• Cat.No.: ACVR1-20H
• Product Overview: N-terminal 6Histagged, recombinant, human ALK2 amino acids 147-end, expressed by baculovirus in Sf21 insect cells. Purified using immobilized metal affinity chromatography.

Specification

• Description: Activins are dimeric growth and differentiation factors which belong to the transforming growth factor-beta (TGF-beta) superfamily of structurally related signaling proteins. Activins signal through a heteromeric complex of receptor serine kinases which include at least two type I ( I and IB) and two type II (II and IIB) receptors. These receptors are all transmembrane proteins, composed of a ligand-binding extracellular domain with cysteine-rich region, a transmembrane domain, and a cytoplasmic domain with predicted serine/threonine specificity. Type I receptors are essential for signaling; and type II receptors are required for binding ligands and for expression of type I receptors. This gene encodes activin A type I receptor which signals a particular transcriptional response in concert with activin type II receptors. Mutations in this gene are associated with fibrodysplasia ossificans progressive.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: 147-end
• Form: 0.834mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 134U/mg, where one unit of ALK2, active activity is defined as 1nmol phosphate incorporated into casein per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 45 kDa
• AA Sequence: 1 MSYYHHHHHH DYDIPTTENL YFQGAMDPEF RKFKRRNQER LNPRDVEYGT IEGLITTNVG 61 DSTLADLLDH SCTSGSGSGL PFLVQRTVAR QITLLECVGK GRYGEVWRGS WQGENVAVKI 121 FSSRDEKSWF RETELYNTVM LRHENILGFI ASDMTSRHSS TQLWLITHYH EMGSLYDYLQ 181 LTTLDTVSCL RIVLSIASGL AHLHIEIFGT QGKPAIAHRD LKSKNILVKK NGQCCIADLG 241 LAVMHSQSTN QLDVGNNPRV GTKRYMAPEV LDETIQVDCF DSYKRVDIWA FGLVLWEVAR 301 RMVSNGIVED YKPPFYDVVP NDPSFEDMRK VVCVDQQRPN IPNRWFSDPT LTSLAKLMKE 361 CWYQNPSARL TALRIKKTLT KIDNSLDKLK TDC
• Purity: Purity 91% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: ACVR1 activin A receptor type 1 [ Homo sapiens (human) ]
• Official Symbol: ACVR1
• Synonyms: ACVR1; activin A receptor type 1; FOP; ALK2; SKR1; TSRI; ACTRI; ACVR1A; ACVRLK2
• Gene ID: 90
• mRNA Refseq: NM_001105.5
• Protein Refseq: NP_001096.1
• MIM: 102576
• UniProt ID: Q04771