Active Recombinant Human ACVR2B(185-488) Protein, N-GST-tagged

• Cat.No.: ACVR2B-14H
• Product Overview: N-Terminal GST-tagged, recombinant, human ActRIIB amino acids 185-488 expressed by baculovirus in Sf21 insect cells. Purified using glutathione agarose.

Specification

• Description: ActRIIB (Activin receptor type-2B) is a type II transmembrane serine/threonine kinase receptor that is involved in the signaling of members of the TGF-β superfamily of cytokines, including activin, myostatin, and growth and differentiation factor 11 (GDF11). ActRIIB signaling has been shown to inhibit muscle growth and promote muscle wasting through the activation of the Smad2/3 signaling pathway and the subsequent repression of muscle-specific genes. Research has suggested that targeting ActRIIB signaling may be a potential therapeutic strategy for the treatment of muscle wasting diseases, such as sarcopenia, cachexia, and muscular dystrophy. Several drugs targeting ActRIIB signaling, including the myostatin inhibitors and selective androgen receptor modulators (SARMs), are currently in clinical development as potential therapies for these conditions.
• Source: Sf21
• Species: Human
• Tag: GST
• Protein length: 185-488 aa
• Form: 0.39 mg/ml of enzyme in 50 mM Tris/HCl pH 7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 9 U/mg, where one unit of ActRIIB activity is defined as 1 nmol phosphate incorporated into 2 mg/ml casein per minute at 30 centigrade with a final ATP concentration of 100 μM.
• Molecular Mass: 61 kDa
• Purity: 90% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 1 year at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: ACVR2B activin A receptor type 2B [ Homo sapiens (human) ]
• Official Symbol: ACVR2B
• Synonyms: ACVR2B; activin A receptor type 2B; HTX4; ACTRIIB; ActR-IIB
• Gene ID: 93
• mRNA Refseq: NM_001106.4
• Protein Refseq: NP_001097.2
• MIM: 602730
• UniProt ID: Q13705