Active Recombinant Human CAMK2G(1-330) Protein, N-His-tagged

• Cat.No.: CAMK2G-39H
• Product Overview: N-terminal 6Histagged, recombinant, CaM Kinase IIγ, amino acids 1–330, expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTA agarose.

Specification

• Description: CaMK IIγ is a Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca(2+)/calmodulin-binding and autophosphorylation, and is involved in sarcoplasmic reticulum Ca(2+) transport in skeletal muscle and may function in dendritic spine and synapse formation and neuronal plasticity. In slow-twitch muscles, it involved in regulation of sarcoplasmic reticulum (SR) Ca(2+) transport and in fast-twitch muscle participates in the control of Ca(2+) release from the SR through phosphorylation of the ryanodine receptor-coupling factor triadin. In the central nervous system, it is involved in the regulation of neurite formation and arborization.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: 1-330 aa
• Form: 3.885mg/ml of enzyme in 50mM Tris/HCl pH8.0, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 20010U/mg, where one unit of CaM Kinase IIγ activity is defined as 1nmol phosphate incorporated into 250μM (KKLNRTLSFAEPG) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 41.2 kDa
• AA Sequence: 1 MSYYHHHHHH DYDIPTTENL YFQGAMDPEF MATTATCTRF TDDYQLFEEL GKGAFSVVRR 61 CVKKTSTQEY AAKIINTKKL SARDHQKLER EARICRLLKH PNIVRLHDSI SEEGFHYLVF 121 DLVTGGELFE DIVAREYYSE ADASHCIHQI LESVNHIHQH DIVHRDLKPE NLLLASKCKG 181 AAVKLADFGL AIEVQGEQQA WFGFAGTPGY LSPEVLRKDP YGKPVDIWAC GVILYILLVG 241 YPPFWDEDQH KLYQQIKAGA YDFPSPEWDT VTPEAKNLIN QMLTINPAKR ITADQALKHP 301 WVCQRSTVAS MMHRQETVEC LRKFNARRKL KGAILTTMLV SRNFSAAKSL LNKKSDGGVK
• Purity: Purity 91.2% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -20 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: CAMK2G calcium/calmodulin dependent protein kinase II gamma [ Homo sapiens (human) ]
• Official Symbol: CAMK2G
• Synonyms: CAMK2G ; calcium/calmodulin dependent protein kinase II gamma; CAMK; CAMKG; MRD59; CAMK-II
• Gene ID: 818
• mRNA Refseq: NM_001222.4
• Protein Refseq: NP_001213.2
• MIM: 602123
• UniProt ID: Q13555