Active Recombinant Human CXCL10 Protein

• Cat.No.: CXCL10-08H-AF647
• Product Overview: Escherichia coli expressed Human CXCL10 protein was labeled with AF647 in vitro.

Specification

• Description: CXCL10 also known as IP-10 is belonging to the CXC chemokine family. It is encoded by the CXCL10 gene. CXCL10 was originally identified in monocytes, endothelial cells and fibroblasts as a responsor to IFN-γ. This chemokine elicits its effects by binding to the cell surface chemokine receptor CXCR3. CXCL10 has been shown to be a chemoattractant for activated T-lymphocytes and monocytes/macrophages. It also has other roles, such as promotion of T cell adhesion to endothelial cells, and inhibition of bone marrow colony formation and angiogenesis. The mouse homologue of human IP-10, Crg-2, has been cloned and shown to share approximately 67 % amino acid sequence identity with human IP-10.
• Source: E. coli
• Species: Human
• Form: Solution
• Bio-activity: Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood T-lymphocytes is in a concentration range of 10-50 ng/ml.
• Molecular Mass: Approximately 8.6 kDa, a single non-glycosylated polypeptide chain containing 77 amino acids.
• Protein Length: 77 aa
• AA Sequence: VPLSRTVRCT CISISNQPVN PRSLEKLEII PASQFCPRVE IIATMKKKGE KRCLNPESKA IKNLLKAVSK EMSKRSP
• Endotoxin: Less than 1 EU/µg of rHuIP-10/CXCL10 as determined by LAL method.
• Purity: >97% by SDS-PAGE and HPLC analysis.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied.
• Concentration: 2.0 mg/mL

Gene Information

• Gene Name: CXCL10 C-X-C motif chemokine ligand 10 [ Homo sapiens (human) ]
• Official Symbol: CXCL10
• Synonyms: C7; IFI10; INP10; IP-10; crg-2; mob-1; SCYB10; gIP-10
• Gene ID: 3627
• mRNA Refseq: NM_001565.4
• Protein Refseq: NP_001556.2
• MIM: 147310
• UniProt ID: P02778