Active Recombinant Human ERBB4(706-991) Protein, N-His-tagged

• Cat.No.: ERBB4-77H
• Product Overview: N-Terminal 6Histagged, recombinant, human ErB4 amino acids 706-991, expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTAagarose.

Specification

• Description: The ERBB family of receptor tyrosine kinases, namely the EGF receptor (EGFR), ERBB2/HER2/Neu, ERBB3, and ERBB4, regulate cellular proliferation, differentiation, and programmed cell death in a growth factor-dependent manner. ERBBs mediate signaling by a large number of growth factors that are structurally related to EGF such as transforming growth factor-a or amphiregulin.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: 706-991 aa
• Form: 2.65mg/ml of enzyme in 50mM Tris/HCl pH7.5, 150mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 451U/mg, where one unit of ErB4 activity is defined as 1nmol phosphate incorporated into 0.1mg/ml poly(Glu, Tyr) (4:1) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 36.1 kDa
• AA Sequence: 1 MSYYHHHHHH DYDIPTTENL YFQGAMGSNQ AQLRILKETE LKRVKVLGSG AFGTVYKGIW 61 VPEGETVKIP VAIKILNETT GPKANVEFMD EALIMASMDH PHLVRLLGVC LSPTIQLVTQ 121 LMPHGCLLEY VHEHKDNIGS QLLLNWCVQI AKGMMYLEER RLVHRDLAAR NVLVKSPNHV 181 KITDFGLARL LEGDEKEYNA DGGKMPIKWM ALECIHYRKF THQSDVWSYG VTIWELMTFG 241 GKPYDGIPTR EIPDLLEKGE RLPQPPICTI DVYMVMVKCW MIDADSRPKF KELAAEFSRM 301 ARDPQRYLVI QGDD
• Purity: Purity 81% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: ERBB4 erb-b2 receptor tyrosine kinase 4 [ Homo sapiens (human) ]
• Official Symbol: ERBB4
• Synonyms: ERBB4; erb-b2 receptor tyrosine kinase 4; HER4; ALS19; p180erbB4
• Gene ID: 2066
• mRNA Refseq: NM_001042599.1
• Protein Refseq: NP_001036064.1
• MIM: 600543
• UniProt ID: Q15303