Active Recombinant Human ERBB4 Protein, hIgG/His-tagged

• Cat.No.: ERBB4-04H
• Product Overview: Recombinant human ErbB4 (26-649aa), fused to hIgG-His-tag at C-terminus, was expressed in HEK293 and purified by using conventional chromatography techniques.

Specification

• Description: This gene is a member of the Tyr protein kinase family and the epidermal growth factor receptor subfamily. It encodes a single-pass type I membrane protein with multiple cysteine rich domains, a transmembrane domain, a tyrosine kinase domain, a phosphotidylinositol-3 kinase binding site and a PDZ domain binding motif. The protein binds to and is activated by neuregulins and other factors and induces a variety of cellular responses including mitogenesis and differentiation. Multiple proteolytic events allow for the release of a cytoplasmic fragment and an extracellular fragment. Mutations in this gene have been associated with cancer. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized.
• Source: HEK293
• Species: Human
• Tag: hIgG/His
• Form: Liquid
• Bio-activity: Measured by its binding ability in a functional ELISA with Human NRG1/HRG1. The ED50 range ≤ 0.2 μg/mL.
• Molecular Mass: 96.6 kDa (863aa)
• AA Sequence: QSVCAGTENKLSSLSDLEQQYRALR KYYENCEVVMGNLEITSIEHNRDLS FLRSVREVTGYVLVALNQFRYLPLE NLRIIRGTKLYEDRYALAIFLNYRK DGNFGLQELGLKNLTEILNGGVYVD QNKFLCYADTIHWQDIVRNPWPSNL TLVSTNGSSGCGRCHKSCTGRCWGP TENHCQTLTRTVCAEQCDGRCYGPY VSDCCHRECAGGCSGPKDTDCFACM NFNDSGACVTQCPQTFVYNPTTFQL EHNFNAKYTYGAFCVKKCPHNFVVD SSSCVRACPSSKMEVEENGIKMCKP CTDICPKACDGIGTGSLMSAQTVDS SNIDKFINCTKINGNLIFLVTGIHG DPYNAIEAIDPEKLNVFRTVREITG FLNIQSWPPNMTDFSVFSNLVTIGG RVLYSGLSLLILKQQGITSLQFQSL KEISAGNIYITDNSNLCYYHTINWT TLFSTINQRIVIRDNRKAENCTAEG MVCNHLCSSDGCWGPGPDQCLSCRR FSRGRICIESCNLYDGEFREFENGS ICVECDPQCEKMEDGLLTCHGPGPD NCTKCSHFKDGPNCVEKCPDGLQGA NSFIFKYADPDRECHPCHPNCTQGC NGPTSHDCIYYPWTGHSTLPQHAR
• Endotoxin: < 1 EU/μg of protein (determined by LAL method)
• Purity: > 90% by SDS-PAGE
• Applications: SDS-PAGE, Bioactivity
• Storage: Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/mL (determined by Absorbance at 280nm)
• Storage Buffer: Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Gene Information

• Gene Name: ERBB4 erb-b2 receptor tyrosine kinase 4 [ Homo sapiens (human) ]
• Official Symbol: ERBB4
• Synonyms: ERBB4; erb-b2 receptor tyrosine kinase 4; HER4; ALS19; p180erbB4; receptor tyrosine-protein kinase erbB-4; avian erythroblastic leukemia viral (v-erb-b2) oncogene homolog 4; human epidermal growth factor receptor 4; proto-oncogene-like protein c-ErbB-4; tyrosine kinase-type cell surface receptor HER4; v-erb-a erythroblastic leukemia viral oncogene homolog 4; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 4; EC 2.7.10.1
• Gene ID: 2066
• mRNA Refseq: NM_005235
• Protein Refseq: NP_005226
• MIM: 600543
• UniProt ID: Q15303