Active Recombinant Human FGFR3(447-761) Protein, N-His-tagged

• Cat.No.: FGFR3-85H
• Product Overview: N-terminal 6Histagged, recombinant, human FGFR3, amino acids 447–761, expressed by baculovirus in SF21 insect cells. Purified using Ni2+/NTA-agarose.

Specification

• Description: Fibroblast growth factor (FGF) superfamily consists of 23 members, all of which contain a conserved 120 amino acid (aa) core region that contains six identical, interspersed amino acids. Based on the pI factor, two main groups of FGF are named: acidic FGF (aFGF) and basic FGF (bFGF). The superfamily members act extracellularly through four tyrosine kinase FGF receptors (FGR1 through FGR4), with multiple specificities noted for almost all FGFs. Biological binding of aFGF to its receptors will result in phosphorylation of the receptor and non-receptor proteins and generate its function. FGR3 (FGFR-3, Fibroblast growth factor receptor 3, EC 2.7.1.112) is expressed mainly in bone, central nervous system and tumors. Inhibition of FGR3 may be useful in the treatment of dwarfism as well as some tumors.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: 447-761 aa
• Form: 0.561mg/ml in 50mM Tris/HCl pH7.5, 150mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 2301U/mg, where one unit of FGFR3, active activity is defined as 1nmol phosphate incorporated into 0.1mg/ml poly(Glu, Tyr) (4:1) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 36.9 kDa
• AA Sequence: 1 MHHHHHHEFE GPTLANVSEL ELPADPKWEL SRARLTLGKP LGEGCFGQVV MAEAIGIDKD 61 RAAKPVTVAV KMLKDDATDK DLSDLVSEME MMKMIGKHKN IINLLGACTQ GGPLYVLVEY 121 AAKGNLREFL RARRPPGLDY SFDTCKPPEE QLTFKDLVSC AYQVARGMEY LASQKCIHRD 181 LAARNVLVTE DNVMKIADFG LARDVHNLDY YKKTTNGRLP VKWMAPEALF DRVYTHQSDV 241 WSFGVLLWEI FTLGGSPYPG IPVEELFKLL KEGHRMDKPA NCTHDLYMIM RECWHAAPSQ 301 RPTFKQLVED LDRVLTVTST DEYL
• Purity: Purity 75.1% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 1 year at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: FGFR3 fibroblast growth factor receptor 3 [ Homo sapiens (human) ]
• Official Symbol: FGFR3
• Synonyms: FGFR3; fibroblast growth factor receptor 3; ACH; CEK2; JTK4; CD333; HSFGFR3EX
• Gene ID: 2261
• mRNA Refseq: NM_000142.5
• Protein Refseq: NP_000133.1
• MIM: 134934
• UniProt ID: P22607