| Description : |
Glycogen synthase kinase-3 (GSK-3) is a protein kinase that was originally identified as a regulator of glycogen synthase, a key enzyme in glycogen metabolism. There are two isoforms of GSK-3, GSK-3a and GSK-3b, and they are strictly regulated via phosphorylation. Phosphorylation of GSK-3b on Ser9 by protein kinase B (PKB) causes its inactivation and is the primary mechanism responsible for growth factor inhibition of this kinase. Activation of GSK-3b is dependent upon the phosphorylation of Tyr216. Upon activation, it has been shown to phosphorylate a number of different cellular proteins, including p53, c-Myc, c-Jun, heat shock factor-1 (HSF-1), and cyclin D1. GSK-3b also has been shown to phosphorylate aberrant sites on the microtubule associated protein tau, which is critical for the progression of Alzheimer's disease. The inhibitors may have therapeutic opportunities for the treatment of diabetes, Alzheimer's disease, stroke and bipolar disorder. EMBL L33801, possessing the amino acid residue substitution H350L. This conflict is reported in the GenBank EST's AI761503 and AL521664, and the March updated version of Swiss Prot P49841. |
| Source : |
Sf21 |
| Species : |
Human |
| Tag : |
His |
| Protein length : |
2-end |
| Form : |
2.153mg/ml of enzyme in 50mM Tris/HCl pH7.5, 150mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 0.2mM PMSF, 1mM benzamidine, 0.1% 2-mercaptoethanol. Frozen solution. |
| Bio-activity : |
808U/mg, where one unit of GSK3β activity is defined as 1nmol phosphate incorporated into 20μM phospho GS2 per minute at 30 centigrade with a final ATP concentration of 100μM. |
| Molecular Mass : |
51 kDa |
| AA Sequence : |
1 MAHHHHHHGS ATMEFMPMES GRPRTTSFAE SCKPVQQPSA FGSMKVSRDK DGSKVTTVVA 61 TPGQGPDRPQ EVSYTDTKVI GNGSFGVVYQ AKLCDSGELV AIKKVLQDKR FKNRELQIMR 121 KLDHCNIVRL RYFFYSSGEK KDEVYLNLVL DYVPETVYRV ARHYSRAKQT LPVIYVKLYM 181 YQLFRSLAYI HSFGICHRDI KPQNLLLDPD TAVLKLCDFG SAKQLVRGEP NVSYICSRYY 241 RAPELIFGAT DYTSSIDVWS AGCVLAELLL GQPIFPGDSG VDQLVEIIKV LGTPTREQIR 301 EMNPNYTEFK FPQIKAHPWT KVFRPRTPPE AIALCSRLLE YTPTARLTPL EACAHSFFDE 361 LRDPNVKLPN GRDTPALFNF TTQELSSNPP LATILIPPHA RIQAAASTPT NATAASDANT 421 GDRGQTNNAA SASASNST |
| Purity : |
Purity 92.9% by SDS-PAGE and Coomassie blue staining. |
| Applications : |
Drug Discovery & Development |
| Storage : |
6 months at -70 centigrade. |
| Handling Advice : |
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade. |
| Use/Stability : |
On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 6 months from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap. |
