Recombinant Mouse GSK3B cell lysate

• Cat.No.: GSK3B-534MCL
• Product Overview: Mouse GSK3B derived in Baculovirus-Insect cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls

Specification

• Source: Baculovirus-Insect Cells
• Species: Mouse
• Preparation method: Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
• Lysis buffer: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Quality control Testing: 12.5% SDS-PAGE Stained with Coomassie Blue
• Recommended Usage: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
• Stability: Samples are stable for up to twelve months from date of receipt at -80°C
• Storage Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Storage Instruction: Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
• Tag: Non

Gene Information

• Gene Name: Gsk3b glycogen synthase kinase 3 beta [ Mus musculus ]
• Official Symbol: GSK3B
• Synonyms: GSK3B; glycogen synthase kinase 3 beta; glycogen synthase kinase-3 beta; GSK-3 beta; serine/threonine-protein kinase GSK3B; GSK3; GSK-3; C86142; GSK-3beta; 7330414F15Rik; 8430431H08Rik
• Gene ID: 56637
• mRNA Refseq: NM_019827
• Protein Refseq: NP_062801
• Pathway: AKT phosphorylates targets in the cytosol, organism-specific biosystem; Adaptive Immune System, organism-specific biosystem; Alzheimers disease, organism-specific biosystem; Alzheimers disease, conserved biosystem; Axon guidance, organism-specific biosystem; Axon guidance, conserved biosystem; Axon guidance, organism-specific biosystem
• Function: ATP binding; NF-kappaB binding; RNA polymerase II transcription factor binding; beta-catenin binding; beta-catenin binding; integrin binding; ionotropic glutamate receptor binding; kinase activity; nucleotide binding; p53 binding; protein binding; protein kinase A catalytic subunit binding; protein kinase activity; protein kinase binding; protein serine/threonine kinase activity; protein serine/threonine kinase activity; protein serine/threonine kinase activity; tau protein binding; tau-protein kinase activity; tau-protein kinase activity; transferase activity; transferase activity, transferring phosphorus-containing groups; ubiquitin protein ligase binding

Reviews

10/19/2022

In response to the problems in the experiment, the technician gave a satisfactory solution.

05/02/2022

High protein concentrations were received and the desired experimental results were obtained.

04/07/2022

GSK3B proteins purchased are as advertised and the quality of the product is guaranteed.

Q&As

What is the relationship between GSK3B expression and the severity of acute edematous pancreatitis development?

GSK3B expression increased with increasing severity of acute edematous pancreatitis.

What is the relationship between GSK3B and the development of cervical and ovarian cancer?

GSK3B may have the effect of promoting the survival and proliferation of cervical and ovarian cancer tumor cells and inhibiting tumor cell apoptosis.

GSK3B is closely related to the study of which diseases?

GSK3B is closely related to the study of cervical cancer, gastric cancer, breast cancer and other diseases.

What biological processes is GSK3B involved in?

GSK3B is involved in the regulation of cell proliferation, differentiation, survival and apoptosis.

Where is GSK3B located in pancreatic tissue?

In pancreatic tissues, GSK3B is localized to the cytoplasm.