Active Recombinant Human JAK2(808-end) Protein, C-His-tagged

• Cat.No.: JAK2-116H
• Product Overview: C-terminal 6Histagged, recombinant, human JAK2, amino acids 808–end, expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTA agarose followed by gel filtration.

Specification

• Description: Janus kinase 2 (JAK2) is a non-receptor tyrosine kinase belonging to the Janus family. It is activated by a variety of cytokine and growth factor receptors, resulting in signaling cascades that facilitate the activation of various downstream target genes. JAK2 mediates gene transcription through its well-characterized downstream signaling molecules, the signal transducers and activators of transcription proteins (STAT).
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: 808-end
• Form: 0.509mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 3627U/mg, where one unit of JAK2, active activity is defined as 1nmol phosphate incorporated into 100μM PDKtide per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 38.9 kDa
• AA Sequence: 1 MLFTPDYELL TENDMLPNMR IGALGFSGAF EDRDPTQFEE RHLKFLQQLG KGNFGSVEMC 61 RYDPLQDNTG EVVAVKKLQH STEEHLRDFE REIEILKSLQ HDNIVKYKGV CYSAGRRNLK 121 LIMEYLPYGS LRDYLQKHKE RIDHIKLLQY TSQICKGMEY LGTKRYIHRD LATRNILVEN 181 ENRVKIGDFG LTKVLPQDKE YYKVKEPGES PIFWYAPESL TESKFSVASD VWSFGVVLYE 241 LFTYIEKSKS PPAEFMRMIG NDKQGQMIVF HLIELLKNNG RLPRPDGCPD EIYMIMTECW 301 NNNVNQRPSF RDLALRVDQI RDNMAGHHHH HH
• Purity: Purity 55.2% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: JAK2 Janus kinase 2 [ Homo sapiens (human) ]
• Official Symbol: JAK2
• Synonyms: JAK2; Janus kinase 2; JTK10
• Gene ID: 3717
• mRNA Refseq: NM_004972.4
• Protein Refseq: NP_004963.1
• MIM: 147796
• UniProt ID: O60674