JAK1

  • Official Full Name

    Janus kinase 1

  • Overview

    Members of the Janus family of tyrosine kinases (Jak1, Jak2, Jak3, and Tyk2) are activated by ligands binding to a number of associated cytokine receptors. Upon cytokine receptor activation, Jak proteins become autophosphorylated and phosphorylate their a
  • Synonyms

    JAK1; Janus kinase 1; JAK1B; tyrosine-protein kinase JAK1; JAK1A; JTK3;

  • Recombinant Proteins
  • Cell & Tissue Lysates
  • Protein Pre-coupled Magnetic Beads
  • Chicken
  • Human
  • Human
  • Mouse
  • Rhesus Macaque
  • Zebrafish
  • Baculovirus-Insect cells
  • E.coli
  • HEK293
  • HEK293T
  • Human cells
  • Insect Cell
  • Insect cells
  • Mammalian Cell
  • Mammalian cells
  • Sf21
  • Sf9
  • Sf9 Insect Cell
  • Yeast
  • Flag
  • GST
  • His
  • Fc
  • Avi
  • MBP
  • T7
  • Myc
  • DDK
  • N
  • 6×His
  • Non
Species Cat.# Product name Source (Host) Tag Protein Length Price
Human JAK1-670H Active Recombinant Human JAK1, GST-tagged Sf9 Insect Cell GST 866-1155
Human JAK1-18H Active Recombinant Human JAK1 protein, GST-tagged Insect Cell GST 438-end
Human JAK1-115H Active Recombinant Human JAK1(866-end) Protein, N-GST-tagged Sf21 GST 866-end
Human JAK1-56H Recombinant Human JAK1 protein, Flag-tagged, Biotinylated Insect Cell Flag 850-1154
Human JAK1-28H Recombinant Human JAK1 protein, MYC/DDK-tagged HEK293 Myc&DDK
Human JAK1-249H Recombinant Human JAK1 Mammalian Cell His
Human JAK1-674H Recombinant Human Janus Kinase 1, GST-tagged Insect Cell GST
Human JAK1-159H Recombinant Human JAK1 protein, His/MBP-tagged E.coli His&MBP 450-618 aa
Human JAK1-160H Recombinant Human JAK1 protein, His/MBP-tagged E.coli His&MBP 1-189 aa
Human JAK1-5107HCL Recombinant Human JAK1 293 Cell Lysate HEK293 Non
Human JAK1-151H Recombinant Human JAK1 Protein, DYKDDDDK-tagged Human cells Flag
Human JAK1-938H Recombinant Human JAK1 protein, His-tagged Insect cells His Ala 561 - Asn 852
Human JAK1-1941H Recombinant Human JAK1 Protein (850-1154 aa), His-tagged Yeast His 850-1154 aa
Human JAK1-3126H Recombinant Human JAK1 protein, His-tagged E.coli His 850-1154aa
Human JAK1-151HFL Recombinant Full Length Human JAK1 Protein, C-Flag-tagged Mammalian cells Flag
Human JAK1-2667H Recombinant Human JAK1 protein, His-tagged E.coli His 1080-1151 aa
Human JAK1-0263H Recombinant Human JAK1 Protein (Ile841-Lys1154), C-His-tagged Baculovirus-Insect cells His Ile841-Lys1154
Human JAK1-1227H Recombinant Human JAK1 Protein, His (Fc)-Avi-tagged HEK293 His&Fc&Avi
Human JAK1-3130H Recombinant Human JAK1 Protein (Pro32-Phe286), N-His tagged E.coli His Pro32-Phe286
Human JAK136418H Recombinant Human JAK1 (849-1154) Protein
Human JAK13406H Recombinant Human JAK1 (854-1154) Protein
Human JAK136170H Recombinant Human JAK1 (865-1154) Protein
Human JAK1-1227H-B Recombinant Human JAK1 Protein Pre-coupled Magnetic Beads HEK293
Human JAK1-07H Recombinant Human Janus kinase 1 Protein, His-tagged Sf9 N-6×His 561-852
Mouse Jak1-7876M Recombinant Mouse Jak1 protein, His & T7-tagged E.coli His&T7 Gln833~Leu1074
Mouse Jak1-3626M Recombinant Mouse Jak1 Protein, Myc/DDK-tagged HEK293T Myc&DDK
Mouse Jak1-3127M Recombinant Mouse Jak1 protein, His-tagged E.coli His 848-1152aa
Rhesus Macaque JAK1-2326R Recombinant Rhesus monkey JAK1 Protein, His-tagged Mammalian Cell His
Rhesus Macaque JAK1-2147R Recombinant Rhesus Macaque JAK1 Protein, His (Fc)-Avi-tagged HEK293 His&Fc&Avi
Rhesus Macaque JAK1-2147R-B Recombinant Rhesus Macaque JAK1 Protein Pre-coupled Magnetic Beads HEK293
Zebrafish JAK1-8699Z Recombinant Zebrafish JAK1 Mammalian Cell His
Chicken JAK1-6433C Recombinant Chicken JAK1 Mammalian Cell His
  • Background
  • Quality Guarantee
  • Case Study
  • Involved Pathway
  • Protein Function
  • Interacting Protein
  • Other Resource
  • JAK1 Related Signal Pathway

What is JAK1 protein?

JAK1 gene (Janus kinase 1) is a protein coding gene which situated on the short arm of chromosome 1 at locus 1p31. This gene encodes a membrane protein that is a member of a class of protein-tyrosine kinases (PTK) characterized by the presence of a second phosphotransferase-related domain immediately N-terminal to the PTK domain. The encoded kinase phosphorylates STAT proteins (signal transducers and activators of transcription) and plays a key role in interferon-alpha/beta, interferon-gamma, and cytokine signal transduction. This gene plays a crucial role in effecting the expression of genes that mediate inflammation, epithelial remodeling, and metastatic cancer progression. This gene is a key component of the interleukin-6 (IL-6)/JAK1/STAT3 immune and inflammation response and is a therapeutic target for alleviating cytokine storms. The JAK1 protein is consisted of 1154 amino acids and JAK1 molecular weight is approximately 133.3 kDa.

What is the function of JAK1 protein?

JAK1 phosphorylates STAT proteins, which are then activated, dimerize, and translocate to the nucleus to regulate the expression of specific genes. JAK1 plays a significant role in immune responses, including the development and function of the immune system, particularly in the signaling of interferons (IFNs) and interleukins (ILs). It contributes to the regulation of cell growth, differentiation, and survival through its involvement in cytokine signaling pathways. JAK1 is involved in various developmental processes, including organogenesis and tissue repair. JAK1 is crucial for blood cell production, as it is involved in the signaling pathways of several cytokines important for hematopoietic function.

JAK1 Related Signaling Pathway

JAK1 plays an important role in the development and function of immune cells, especially in lymphocyte signaling. JAK1 is involved in signaling by binding to specific domains of cytokine receptors via FERM domains and SH2 domains at its amino terminus. JAK1 mediates the transmission of various cytokine signals, such as interferon (IFNs) and interleukin (ILs), which play a key role in cell proliferation, differentiation, apoptosis and immune regulation. JAK1 can phosphorylate STAT protein after activation, resulting in the dimerization of STAT protein and migration to the nucleus, regulating the expression of target genes.

JAK1 Related Diseases

Mutations in the JAK1 gene can lead to primary immunodeficiency, such as some types of severe combined immunodeficiency (SCID), because JAK1 plays a crucial role in the development and function of lymphocytes. Overactivation of JAK1 has been linked to the development of autoimmune diseases such as rheumatoid arthritis, psoriasis, and inflammatory bowel disease, which involve the immune system attacking its own tissues. Sustained activation of JAK1 is associated with the development, progression, and treatment resistance of certain cancers, including blood cancers and solid tumors, and it may promote the proliferation, survival, and metastasis of tumor cells. JAK1's role in regulating the immune response also makes it relevant for allergic diseases, such as asthma, where activation of JAK1 can lead to inflammation and activation of immune cells. In addition, it is also associated with viral infection, chronic inflammation and blood diseases.

JAK1-7.jpg

Fig1. JAK/STAT pathway in myeloproliferative neoplasms. (Alfonso Quintás-Cardama, 2013)

Bioapplications of JAK1

In terms of disease treatment, as a key component of the JAK/STAT signaling pathway, JAK1 is a focus for the development of targeted therapeutics for the treatment of autoimmune diseases, inflammation, and certain cancers. JAK1 inhibitors are used to regulate the immune response and reduce the activity of pathological immune cells and inflammation in the treatment of autoimmune diseases. The active status of JAK1 or its mutation may be used as a disease marker for the diagnosis of certain diseases and the monitoring of therapeutic response. In basic research, JAK1 serves as an important tool for studying cell signaling, immune response, and disease mechanisms, contributing to a deeper understanding of related biological processes.

High Purity

SDS-PAGE (JAK1-938H).jpg

Fig1. SDS-PAGE (JAK1-938H)

High Bioactivity

Activity Data (JAK1-115H).jpg

Fig2. Activity Data (JAK1-115H)

Case Study 1: Nicholas P D Liau, 2018

Interferon gamma (IFNγ) is a potent inflammatory and immune cytokine. IFNγ signals via the interferon gamma receptor (IFNGR), which is constitutively bound to Janus Kinase (JAK) 1 and JAK2 via its intracellular domain. These two JAK proteins then initiate the inflammatory signaling cascade. The most potent inhibitor of IFNγ signaling is Suppressor of Cytokine Signaling 1 (SOCS1). SOCS1 negatively regulates IFNγ signaling pathway (and other pathways) by directly inhibiting JAKs. In this study, purified JAK1 was used for structural and biochemical studies.

JAK1-1.jpg

Fig1. SDS-PAGE gel stained with Coomassie Blue.

JAK1-2.jpg

Fig2. A 280 absorbance trace of JAK1 JH1 elution from a Superdex200 16/600 size exclusion chromatography column.

Case Study 2: Yuechao Sun, 2023

The role of METTL3-mediated N6-methyladenosine (m6A) modification has been elucidated in several cancers, but the concrete mechanism underlying its function in colorectal cancer is still obscure. Here, researchers revealed that upregulated methyltransferase-like 3 (METTL3) in colorectal cancer exerted both methyltransferase activity-dependent and -independent functions in gene regulation. METTL3 deposited m6A on the 3' untranslated region of the JAK1 transcript to promote JAK1 translation relying on YTHDF1 recognition. The increased JAK1 and STAT3 corporately contributed to the activation of the p-STAT3 signaling pathway and further upregulated downstream effectors expressions, including VEGFA and CCND1, which finally resulted in enhanced cancer cell proliferation and metastasis in vitro and in vivo.

JAK1-3.jpg

Fig3. METTL3-regulated JAK1 expression is independent of proteasome-mediated protein degradation.

JAK1-4.jpg

Fig4. Dual-luciferase reporter assays for the wild type or mutant JAK1 3'UTR sequence in cancer cells with or without silenced YTHDF1.

JAK1 involved in several pathways and played different roles in them. We selected most pathways JAK1 participated on our site, such as ARMS-mediated activation, Antiviral mechanism by IFN-stimulated genes, Axon guidance, which may be useful for your reference. Also, other proteins which involved in the same pathway with JAK1 were listed below. Creative BioMart supplied nearly all the proteins listed, you can search them on our site.

Pathway Name Pathway Related Protein
ARMS-mediated activationSPRED3;JAK3;NEFLB;RASGRP1;AKAP9;SLC14A2;FGF10B;FGF20A;FRS2A
Antiviral mechanism by IFN-stimulated genesEIF4A2;KPNA3;ARIH1L;IFIT14;HERC5;IFIT12;EIF4G1;IFIT11;ARIH1
Axon guidanceNETO2;ROCK2;EPHA4A;NADL1.1;EGF;SCN4A;DUSP10;CDC42;DCX
Cytokine Signaling in Immune systemFRS2B;EIF4E3;TRIM29;DUSP2;SPRED1;KBTBD7;DUSP10;CSF2RA;IRAK3
DAP12 interactionsPRKAR1AA;RASGEF1A;DUSP4;ADRBK1;DUSP2;HBEGF;MOV10;FRS3;PAQR3B
DAP12 signalingFRS2B;FRS3;DUSP7;AGO3;SPTB;DUSP6;PEA15;FGF18B;RASGRP1
Developmental BiologyAP2M1;CACNG2;DPYSL5B;CEBPA;COL4A5;LGI2A;ADAM22;DUSP6;COL6A3
Downstream signal transductionRASGEF1A;FGF18B;PAQR3B;MOV10;CNKSR1;LAMTOR2;PTPRA;KBTBD7;SPTB

JAK1 has several biochemical functions, for example, ATP binding, CCR5 chemokine receptor binding, growth hormone receptor binding. Some of the functions are cooperated with other proteins, some of the functions could acted by JAK1 itself. We selected most functions JAK1 had, and list some proteins which have the same functions with JAK1. You can find most of the proteins on our site.

Function Related Protein
ATP bindingABCB6;LONP1;IKBKE;PRKCZ;PL10;PDXK;ABCF2;ERCC6L2;MAPK12A
CCR5 chemokine receptor bindingCCL4;STAT3;CNIH4;JAK1;CCL3;CCL5
growth hormone receptor bindingJAK1;Gh;SOCS2;GH1;TYK2;JAK2
non-membrane spanning protein tyrosine kinase activityFYNB;JAK2B;PTK2BB;TNK2B;STYK1;ITK;MELK;FYNA;ZNF143B
protein bindingPOC1B;PTCRA;HTR6;DCAF7;PPARD;HINT2;CYB5R2;ROCK2A;NRXN1
protein phosphatase bindingPARD3;SNX3;ERBB2;VCAN;FLT4;ANAPC5;VRK3;EGFR;CTNNB1
protein tyrosine kinase activityPTK6B;SRC;LYN;IGF1R;FER;KDRL;DYRK3;FGR;ALK
ubiquitin protein ligase bindingUBE2R2;TRIM37;UBE2C;FAF1;SKI;TRIB2;SMAD6;TNFRSF14;UBE2L3

JAK1 has direct interactions with proteins and molecules. Those interactions were detected by several methods such as yeast two hybrid, co-IP, pull-down and so on. We selected proteins and molecules interacted with JAK1 here. Most of them are supplied by our site. Hope this information will be useful for your research of JAK1.

IFNAR2; STAM2; ERBB2; Dlg4; P; v_rindsa; HSP90AB1; IL6R; PRKCA; BRCA1; P/V; V

Gene Family

TGF Cytokines

Research Area

Related articles

Cianciulli, A; Dragone, T; et al. IL-10 plays a pivotal role in anti-inflammatory effects of resveratrol in activated microglia cells. INTERNATIONAL IMMUNOPHARMACOLOGY 24:369-376(2015).
Kubo, S; Yamaoka, K; et al. The JAK inhibitor, tofacitinib, reduces the T cell stimulatory capacity of human monocyte-derived dendritic cells. ANNALS OF THE RHEUMATIC DISEASES 73:2192-2198(2014).
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Customer Reviews (3)

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03/13/2022

    Top-notch purity, impressive results.

    09/30/2021

      Excellent stability, very useful.

      11/17/2020

        Fast and reliable delivery.

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