Active Recombinant Human KIT(544-end) Protein, N-GST-tagged

• Cat.No.: KIT-36H
• Product Overview: N-terminal GST tagged, recombinant, human Kit, amino acids 544–end, expressed by baculovirus, in Sf21 insect cells. Purified using glutathione agarose.

Specification

• Description: Binding of stem cell factor (SCF) to C-Kit receptor tyrosine kinase induces homodimeriziaton and intermolecular tyrosine phosphorylation of the receptor, creating docking sites for a number of SH2-containing signal transduction molecules. These include phosphatidylinositol 3'-kinase (PI 3K) and phospholipase C-1, Stat1, the Src family kinases Src, Lyn, and Fyn, and the GTPase activating protein GAP. Mutation in Kit which result in ligand-independent activation of the kinase were found to be associated with human gastrointestinal stromal tumors and mastocytosis.
• Source: Sf21
• Species: Human
• Tag: GST
• Protein length: 544-end
• Form: 0.883mg/ml of enzyme in 50mM Tris/HCl pH7.5, 150mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 33U/mg, where one unit of Kit activity is defined as 1nmol phosphate incorporated into 0.1mg/ml poly(Glu, Tyr) (4:1) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 76.7 kDa
• AA Sequence: 1 MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID 61 GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV 121 DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK 181 KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LEVLFQGPEF KGLRRQTYKY 241 LQKPMYEVQW KVVEEINGNN YVYIDPTQLP YDHKWEFPRN RLSFGKTLGA GAFGKVVEAT 301 AYGLIKSDAA MTVAVKMLKP SAHLTEREAL MSELKVLSYL GNHMNIVNLL GACTIGGPTL 361 VITEYCCYGD LLNFLRRKRD SFICSKQEDH AEAALYKNLL HSKESSCSDS TNEYMDMKPG 421 VSYVVPTKAD KRRSVRIGSY IERDVTPAIM EDDELALDLE DLLSFSYQVA KGMAFLASKN 481 CIHRDLAARN ILLTHGRITK ICDFGLARDI KNDSNYVVKG NARLPVKWMA PESIFNCVYT 541 FESDVWSYGI FLWELFSLGS SPYPGMPVDS KFYKMIKEGF RMLSPEHAPA EMYDIMKTCW 601 DADPLKRPTF KQIVQLIEKQ ISESTNHIYS NLANCSPNRQ KPVVDHSVRI NSVGSTASSS 661 QPLLVHDDV
• Purity: Purity 86.7% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -20 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: KIT KIT proto-oncogene, receptor tyrosine kinase [ Homo sapiens (human) ]
• Official Symbol: KIT
• Synonyms: KIT; KIT proto-oncogene, receptor tyrosine kinase; PBT; SCFR; C-Kit; CD117; MASTC
• Gene ID: 3815
• mRNA Refseq: NM_000222.3
• Protein Refseq: NP_000213.1
• MIM: 164920
• UniProt ID: P10721