Active Recombinant Rhesus KIT protein(Met1-Thr520), His-tagged

• Cat.No.: KIT-2409R
• Product Overview: Recombinant Rhesus KIT (NP_001253024.1) (Met1-Thr520) was expressed in HEK293 with a polyhistidine tag at the C-terminus.

Specification

• Species: Rhesus macaque
• Source: HEK293
• Tag: His
• Protein Length: Met1-Thr520
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Immobilized Recombinant Rhesus c-Kit Protein (ECD, His Tag) at 2μg/mL (100μL/well) can bind Recombinant Human SCF / C-kit ligand Protein (aa 1-189, His & AVI Tag), Biotinylated, the EC50 is 1.3-4.6 ng/mL.
• Molecular Mass: The recombinant Rhesus KIT consists of 506 amino acids and predicts a molecular mass of 57 kDa. It migRates as an approximately 93.3 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: ≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.