| Description : |
This protein is a member of the leucine-rich repeat kinase family and encodes a protein with an ankryin repeat region, a leucine-rich repeat (LRR) domain, a kinase domain, a DFG-like motif, a RAS domain, a GTPase domain, a MLK-like domain, and a WD40 domain. The protein is present largely in the cytoplasm but also associates with the mitochondrial outer membrane. Mutations in this gene have been associated with Parkinson disease-8. |
| Source : |
Sf21 |
| Species : |
Human |
| Tag : |
Flag |
| Protein length : |
970-end |
| Form : |
0.166mg/ml of enzyme in 50mM Tris/HCl pH7.4, 300mM NaCl, 20mM β-glycerophosphate, 0.25mM sodium orthovanadate, 10mM NaF, 150μg/ml FLAG peptide, 20% v/v glycerol, 1mM benzamidine, 0.2mM PMSF, 5mM DTT. Frozen solution. |
| Bio-activity : |
147U/mg, where one unit of LRRK2, active activity is defined as 1nmol phosphate incorporated into 250μM ERMtide (RLGRDKYKTLRQIRQ) per minute at 30 centigrade with a final ATP concentration of 100μM. |
| Molecular Mass : |
180 kDa |
| AA Sequence : |
1 MGGDYKDDDD KDRSHNQTSL YKKAGTHSDS ISSLASEREY ITSLDLSANE LRDIDALSQK 61 CCISVHLEHL EKLELHQNAL TSFPQQLCET LKSLTHLDLH SNKFTSFPSY LLKMSCIANL 121 DVSRNDIGPS VVLDPTVKCP TLKQFNLSYN QLSFVPENLT DVVEKLEQLI LEGNKISGIC 181 SPLRLKELKI LNLSKNHISS LSENFLEACP KVESFSARMN FLAAMPFLPP SMTILKLSQN 241 KFSCIPEAIL NLPHLRSLDM SSNDIQYLPG PAHWKSLNLR ELLFSHNQIS ILDLSEKAYL 301 WSRVEKLHLS HNKLKEIPPE IGCLENLTSL DVSYNLELRS FPNEMGKLSK IWDLPLDELH 361 LNFDFKHIGC KAKDIIRFLQ QRLKKAVPYN RMKLMIVGNT GSGKTTLLQQ LMKTKKSDLG 421 MQSATVGIDV KDWPIQIRDK RKRDLVLNVW DFAGREEFYS THPHFMTQRA LYLAVYDLSK 481 GQAEVDAMKP WLFNIKARAS SSPVILVGTH LDVSDEKQRK ACMSKITKEL LNKRGFPAIR 541 DYHFVNATEE SDALAKLRKT IINESLNFKI RDQLVVGQLI PDCYVELEKI ILSERKNVPI 601 EFPVIDRKRL LQLVRENQLQ LDENELPHAV HFLNESGVLL HFQDPALQLS DLYFVEPKWL 661 CKIMAQILTV KVEGCPKHPK GIISRRDVEK FLSKKRKFPK NYMTQYFKLL EKFQIALPIG 721 EEYLLVPSSL SDHRPVIELP HCENSEIIIR LYEMPYFPMG FWSRLINRLL EISPYMLSGR 781 ERALRPNRMY WRQGIYLNWS PEAYCLVGSE VLDNHPESFL KITVPSCRKG CILLGQVVDH 841 IDSLMEEWFP GLLEIDICGE GETLLKKWAL YSFNDGEEHQ KILLDDLMKK AEEGDLLVNP 901 DQPRLTIPIS QIAPDLILAD LPRNIMLNND ELEFEQAPEF LLGDGSFGSV YRAAYEGEEV 961 AVKIFNKHTS LRLLRQELVV LCHLHHPSLI SLLAAGIRPR MLVMELASKG SLDRLLQQDK 1021 ASLTRTLQHR IALHVADGLR YLHSAMIIYR DLKPHNVLLF TLYPNAAIIA KIADYGIAQY 1081 CCRMGIKTSE GTPGFRAPEV ARGNVIYNQQ ADVYSFGLLL YDILTTGGRI VEGLKFPNEF 1141 DELEIQGKLP DPVKEYGCAP WPMVEKLIKQ CLKENPQERP TSAQVFDILN SAELVCLTRR 1201 ILLPKNVIVE CMVATHHNSR NASIWLGCGH TDRGQLSFLD LNTEGYTSEE VADSRILCLA 1261 LVHLPVEKES WIVSGTQSGT LLVINTEDGK KRHTLEKMTD SVTCLYCNSF SKQSKQKNFL 1321 LVGTADGKLA IFEDKTVKLK GAAPLKILNI GNVSTPLMCL SESTNSTERN VMWGGCGTKI 1381 FSFSNDFTIQ KLIETRTSQL FSYAAFSDSN IITVVVDTAL YIAKQNSPVV EVWDKKTEKL 1441 CGLIDCVHFL REVTVKENKE SKHKMSYSGR VKTLCLQKNT ALWIGTGGGH ILLLDLSTRR 1501 LIRVIYNFCN SVRVMMTAQL GSLKNVMLVL GYNRKNTEGT QKQKEIQSCL TVWDINLPHE 1561 VQNLEKHIEV RKELAEKMRR TSVE |
| Purity : |
Purity 73% by SDS-PAGE and Coomassie blue staining. |
| Applications : |
Drug Discovery & Development |
| Storage : |
6 months at -70 centigrade. |
| Handling Advice : |
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade. |
| Use/Stability : |
On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap. |
