Active Recombinant Human MYLK3(1425-1771) Protein, N-His-tagged

• Cat.No.: MYLK3-151H
• Product Overview: N-terminal 6Histagged, recombinant, human MLCK, amino acids 1425–1771, expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTA agarose.

Specification

• Description: MYLK (myosin light polypeptide kinase; myosin light chain kinase, MLCK), which belongs to the family of Ca2+/calmodulin (CaM)-dependent protein kinases, induces a high degree of myosin motor activity by specifically phosphorylating the myosin II regulatory light chain (MRLC). Myosin II phosphorylation is involved in smooth muscle contraction and cell motility in non-muscle cells.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: 1425-1771 aa
• Form: 1.76mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 224U/mg, where one unit of MLCK activity is defined as 1nmol phosphate incorporated into 250μM (KKLNRTLSFAEPG) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 43.7 kDa
• AA Sequence: 1 MSYYHHHHHH DYDIPTTENL YFQGAMDPEF GEKPEEPKDE VEVSDDDEKE PEVDYRTVTI 61 NTEQKVSDFY DIEERLGSGK FGQVFRLVEK KTRKVWAGKF FKAYSAKEKE NIRQEISIMN 121 CLHHPKLVQC VDAFEEKANI VMVLEIVSGG ELFERIIDED FELTERECIK YMRQISEGVE 181 YIHKQGIVHL DLKPENIMCV NKTGTRIKLI DFGLARRLEN AGSLKVLFGT PEFVAPEVIN 241 YEPIGYATDM WSIGVICYIL VSGLSPFMGD NDNETLANVT SATWDFDDEA FDEISDDAKD 301 FISNLLKKDM KNRLDCTQCL QHPWLMKDTK NMEAKKLSKD RMKKYMARRK WQKTGNAVRA 361 IGRLSSMAMI SGLSGRK
• Purity: Purity 88.9% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 6 months from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: MYLK3 myosin light chain kinase 3 [ Homo sapiens (human) ]
• Official Symbol: MYLK3
• Synonyms: MYLK3; myosin light chain kinase 3; MLCK; MLCK2; caMLCK
• Gene ID: 91807
• mRNA Refseq: NM_182493.3
• Protein Refseq: NP_872299.2
• MIM: 612147
• UniProt ID: Q32MK0