Active Recombinant Human PAK1(150-end) Protein, N-Flag-tagged

• Cat.No.: PAK1-178H
• Product Overview: N-terminal FLAGtagged, recombinant, human PAK1 amino acids 150-end, expressed by baculovirus in Sf21 insect cells. Purified by immunoaffinity chromatography.

Specification

• Description: This protein is a family member of serine/threonine p21-activating kinases, known as PAK proteins. These proteins are critical effectors that link to cytoskeleton reorganization and nuclear signaling, and they serve as targets for the small GTP binding proteins Cdc42 and Rac. This specific family member regulates cell motility and morphology. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
• Source: Sf21
• Species: Human
• Tag: Flag
• Protein length: 150-end
• Form: 0.170mg/ml of enzyme in 50mM Tris/HCl pH7.5, 150mM NaCl, 10mM β-glycerophosphate, 1mM sodium orthovanadate, 1mM NaF, 28% v/v glycerol, 0.03% Brij-35, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 45501U/mg, where one unit of PAK1, active activity is defined as 1nmol phosphate incorporated into 200μM (RRRLSFAEPG) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 46 kDa
• AA Sequence: 1 MGGDYKDDDD KDPEFAEDYN SSNALNVKAV SETPAVPPVS EDEDDDDDDA TPPPVIAPRP 61 EHTKSVYTRS VIEPLPVTPT RDVATSPISP TENNTTPPDA LTRNTEKQKK KPKMSDEEIL 121 EKLRSIVSVG DPKKKYTRFE KIGQGASGTV YTAMDVATGQ EVAIKQMNLQ QQPKKELIIN 181 EILVMRENKN PNIVNYLDSY LVGDELWVVM EYLAGGSLTD VVTETCMDEG QIAAVCRECL 241 QALEFLHSNQ VIHRDIKSDN ILLGMDGSVK LTDFGFCAQI TPEQSKRSTM VGTPYWMAPE 301 VVTRKAYGPK VDIWSLGIMA IEMIEGEPPY LNENPLRALY LIATNGTPEL QNPEKLSAIF 361 RDFLNRCLEM DVEKRGSAKE LLQHQFLKIA KPLSSLTPLI AAAKEATKNN H
• Purity: Purity 74% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: PAK1 p21 (RAC1) activated kinase 1 [ Homo sapiens (human) ]
• Official Symbol: PAK1
• Synonyms: PAK1; p21 (RAC1) activated kinase 1; IDDMSSD; p65-PAK; PAKalpha; alpha-PAK
• Gene ID: 5058
• mRNA Refseq: NM_001128620.2
• Protein Refseq: NP_001122092.1
• MIM: 602590
• UniProt ID: Q13153