||Recombinant human HDHD3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
||HDHD3, also known as haloacid dehalogenase-like hydrolase domain-containing protein 3, belongs to the HAD-like hydrolase superfamily. This family of hydrolase enzymes includes L-2-haloacid dehalogenase, epoxide hydrolases and phosphatases.
||Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol.
||32.2 kDa (287aa) confirmed by MALDI-TOF.
||> 95% by SDS - PAGE.
||1.0 mg/ml (determined by Bradford assay).
|Sequences Of Amino Acids:
||MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMAHR LQIRLLTWDV KDT LLRLRHP LGEAYATKAR AHGLEVEPSA LEQGFRQAYRAQSHSFPNYG LSH GLT SRQW WLDVVLQTFH LAGVQDAQAV APIAEQLYKD FSHPCTWQVL DGA EDT LREC RTRGLRLAVI SNFDRRLEGILEGLGLREHF DFVLTSEAAG WPKPDPRIFQ EAL RLAHMEP VVAAHVGDNY LCDYQGPRAV GMHSFLVVGP QALDPVVRDS VPK EH ILPSLAHLLPALDCL EGSTPGL.
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.