Recombinant Human Serpin Peptidase Inhibitor, Clade E (nexin, plasminogen activator inhibitor type 1), Member 1, Cys-tagged

• Cat.No.: SERPINE1-5494H
• Product Overview: Recombinant Human SERPINE1 NBD P9Mutant was created by mutagenesis of the P9 serine residue (Ser338) on thereactive center loop to cysteine. This then provided a free thiol group forincorporation of N, N"-dimethyl-N-(acetyl)-N"-(7-nitrobenz-2-oxa-1, 3-diazol-4-yl)(NBD), a fluorescent probe highly sensitive to changes in solvation andhydrophobic environment.

Specification

• Cat. No.: SERPINE1-5494H
• Description: SERPINE1is a protein that in humans is encoded by the SERPINE1 gene. SERPINE1 is aserine protease inhibitor (serpin) that functions as the principal inhibitorof tissue plasminogen activator (tPA) and urokinase (uPA), the activators ofplasminogen and hence fibrinolysis (the physiological breakdown of bloodclots). It is a serine protease inhibitor (serpin) protein.
• Source: E. coli
• Species: Human
• Form: Frozenliquid in 0.05 M sodium phosphate + 0.1 M NaCl + 1 mM EDTA, pH 6.6.
• Concentration: 2.5 mg/ml
• Molecular Weight: 43.0 kDa
• Storage: Store inworking aliquots at -80°C, protected from light. Avoid repeated freeze-thawcycles.
• OfficialSymbol: SERPINE1

Gene Information

• Gene Name: SERPINE1serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitortype 1), member 1 [ Homo sapiens ]
• Synonyms: SERPINE1; serpin peptidase inhibitor, cladeE (nexin, plasminogen activator inhibitor type 1), member 1; Plasminogen activator inhibitor 1; Endothelialplasminogen activator inhibitor; PAIE; Clade E; PLANH1; SERPINE 1; PAI;PAI-1; PAI1; plasminogen activator inhibitor-1; plasminogen activatorinhibitor, type I; serine (or cysteine) proteinase inhibitor, clade E (nexin,plasminogen activator inhibitor type 1), member 1; Endothelial plasminogenactivator inhibitor
• Gene ID: 5054
• mRNA Refseq: NM_000602
• Protein Refseq: NP_000593
• MIM: 173360
• UniProt ID: P05121
• Chromosome Location: 7q21.3-q22
• Pathway: Adipogenesis; BMAL1:CLOCK/NPAS2 Activates Gene Expression; Blood Clotting Cascade; Chagas disease(American trypanosomiasis); Circadian Clock; Complement and CoagulationCascades; Direct p53 effectors; Dissolution of Fibrin Clot; E2F transcriptionfactor network; HIF-1-alpha transcription factor network; HIF-2-alphatranscription factor network; Hemostasis; Platelet activation; Plateletdegranulation; Regulation of nuclear SMAD2/3 signaling
• Function: peptidase inhibitor activity;protease binding; protein binding; serine-type endopeptidase inhibitoractivity; contributes to receptor binding

Reviews

06/02/2022

It has a wide range of applications in the field of scientific research and is suitable for a variety of experimental design and research purposes.

07/16/2021

When used for experiments, the experimental conditions and results are highly repeatable.

02/28/2021

SERPINE1 has good solubility and is easy to operate and purify.

Q&As

Are there any gene polymorphisms or mutations in SERPINE1, and how do these mutations affect function?

Yes, there are some genetic polymorphisms and mutations SERPINE1. These mutations may affect the structure and function of the protein they encode, which in turn affects their physiological and pathological effects. Some mutations have been shown to be involved in thrombosis and tumorigenesis.

What are the potential therapeutic applications of SERPINE1 inhibitors or activators?

Inhibitors or activators targeting SERPINE1 may have potential applications for the treatment of a variety of diseases, including tumors, thrombosis, neurological diseases, etc. At present, there are some small molecule inhibitors for SERPINE1 that have entered the clinical trial stage.

How does SERPINE1 affect apoptosis and repair of nerve cells?

SERPINE1 may prevent neuronal apoptosis by inhibiting serine protease activity, and may promote neuronal cell repair by regulating the activity of cytokines and growth factors.

What are the new directions or breakthroughs in the study of SERPINE1 and its related molecular mechanisms in future research?

The research on SERPINE1 and its related molecular mechanisms will continue to explore its mechanism of action in human physiological and pathological processes. Future research may uncover new modes of action, new modulators, and new therapeutic applications.

Are there other inhibitors or activators in the SERPINE1?

In addition to being an inhibitor of serine proteases, SERPINE1 may also be affected by other inhibitors or activators. These inhibitors or activators may work by modulating the activity or expression levels of the SERPINE1.

How is SERPINE1 related to Alzheimer's disease?

In Alzheimer's disease, SERPINE1 may influence the onset and progression of the disease by regulating β amyloid deposition and neuroinflammatory processes.