||Recombinant humanECHDC1 protein, fused to His-tag at N-terminus, was expressed in E. coli andpurified by using conventional chromatography.
||ECHDC1,also known as enoyl CoA hydratase domain containing 1, belongs to theenoyl-CoA hydratase/isomerase family. Enoyl-CoA hydratase is an enzyme thathydrates the double bond between the second and third carbons on acyl-CoA.This enzyme is essential to metabolizing fatty acids to produce both acetylCoA and energy.
||Liquid. 20mMTris-HCl buffer (pH8.0) containing 10% glycerol 1mM DTT, 0.1M NaCl.
||35.8kDa(327aa) confirmed by MALDI-TOF.
||> 95%by SDS-PAGE
||1.0mg/ml (determined by Bradford assay)
|Sequences of aminoacids:
||MGSSHHHHHHSSGLVPRGSH MALKQEMAKS LLKTASLSGR TKLLHQTGLS LYSTSHGFYE EEVKKTLQQFPGGSIDLQKE DNGIGILTLN NPSRMNAFSG VMMLQLLEKV IELENWTEGKGLIVRGAKNT FSSGSDLNAV KSLGTPEDGM AVCMFMQNTL TRFMRLPLIS VALVQGWALG GGAEFTTACDFRLMTPESKI RFVHKEMGII PSWGGTTRLV EIIGSRQALK VLSGALKLDS KNALNIGMVE EVLQSSDETKSLEEAQEWLKQFIQGPPEVIRALKKSVCSG RELYLEEALQ NERDLLGTVW GGPANLEAIA KKGKFNK
||Can bestored at +4°C short term (1-2 weeks). For long term storage, aliquot andstore at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
||Fatty Acid Biosynthesis