Cat. No.: |
ECHDC1-1924H |
Product Overview: |
Recombinant humanECHDC1 protein, fused to His-tag at N-terminus, was expressed in E. coli andpurified by using conventional chromatography. |
Description: |
ECHDC1,also known as enoyl CoA hydratase domain containing 1, belongs to theenoyl-CoA hydratase/isomerase family. Enoyl-CoA hydratase is an enzyme thathydrates the double bond between the second and third carbons on acyl-CoA.This enzyme is essential to metabolizing fatty acids to produce both acetylCoA and energy. |
Form: |
Liquid. 20mMTris-HCl buffer (pH8.0) containing 10% glycerol 1mM DTT, 0.1M NaCl. |
Molecular Weight: |
35.8kDa(327aa) confirmed by MALDI-TOF. |
Purity: |
> 95%by SDS-PAGE |
Concentration: |
1.0mg/ml (determined by Bradford assay) |
Sequences of aminoacids: |
MGSSHHHHHHSSGLVPRGSH MALKQEMAKS LLKTASLSGR TKLLHQTGLS LYSTSHGFYE EEVKKTLQQFPGGSIDLQKE DNGIGILTLN NPSRMNAFSG VMMLQLLEKV IELENWTEGKGLIVRGAKNT FSSGSDLNAV KSLGTPEDGM AVCMFMQNTL TRFMRLPLIS VALVQGWALG GGAEFTTACDFRLMTPESKI RFVHKEMGII PSWGGTTRLV EIIGSRQALK VLSGALKLDS KNALNIGMVE EVLQSSDETKSLEEAQEWLKQFIQGPPEVIRALKKSVCSG RELYLEEALQ NERDLLGTVW GGPANLEAIA KKGKFNK |
Storage: |
Can bestored at +4°C short term (1-2 weeks). For long term storage, aliquot andstore at -20°C or -70°C. Avoid repeated freezing and thawing cycles. |
Pathways: |
Fatty Acid Biosynthesis |