Active Recombinant Mouse Pdgfc protein(Val235-Gly345), hFc-tagged

• Cat.No.: Pdgfc-873M
• Product Overview: Recombinant Mouse PDGFC (Q8CI19) (Val 235-Gly 345) was expressed in HEK293, fused with the Fc region of Human IgG1 at the N-terminus.

Specification

• Source: HEK293
• Species: Mouse
• Tag: N-hFc
• Protein length: Val235-Gly345
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: 1. Measured by its binding ability in a functional ELISA. 2. Immobilized mouse PDGF-C at 10 μg/mL (100 μl/well) can bind human PDGFRA, The EC50 of human PDGFRA is 0.71 μg/mL. 3. Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. The ED50 for this effect is typically 0.5-2 μg/mL.
• Molecular Mass: The recombinant mouse PDGFC/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 372 amino acids and has a predicted molecular mass of 40.9 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmPDGFC/Fc monomer is approximately 46 kDa.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 80 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Pdgfc platelet-derived growth factor, C polypeptide [ Mus musculus ]
• Official Symbol: Pdgfc
• Synonyms: PDGFC; platelet-derived growth factor, C polypeptide; platelet-derived growth factor C; SCDGF; VEGF-E; fallotein; spinal cord-derived growth factor; PDGF-C; AI647969; 1110064L01Rik
• Gene ID: 54635
• mRNA Refseq: NM_019971
• Protein Refseq: NP_064355