Active Recombinant Human GDNF

• Cat.No.: GDNF-985H
• Product Overview: The recombinant human G-DNF expressed in CHO cells is disulfide-linked homo-dimer, with an apparent molecular weight of ~30.4 kDa.

Specification

• Description: Glial cell line-derived neurotrophic factor (G-DNF) is a neurotrophic factors belong to TGF-beta super family necessary for neuron survival and phenotypic maintenance in central and peripheral nervous systems. G-DNF has the potent to support the differentiation and survival of many neuron subpopulations, prominent for dopaminergic neurons and motor neurons, as well as Purkinje cells and sympathetic neurons. Sertoli cells, type 1 astrocytes, Schwann cells, neurons, pinealocytes and skeletal muscle cells are known to express GDNF in human. GDNF has shown to interact with GFRA2 and GDNF family receptor alpha 1. Mutations in this gene may be associated with Hirschsprungs disease, Parkinsons disease and amyotrophic lateral sclerosis (ALS).
• Source: CHO Cells
• Species: Human
• Form: Lyophilized after extensive dialysis against PBS.
• Bio-activity: ED50< 1 μg/ml, measured in a cell proliferation assay using rat c6 cells, corresponding to a specific activity of>1 x 103 units/mg
• Molecular Mass: 30.4 kDa (homo-dimer), observed by non-reducing SDS-PAGE.
• AA Sequence: Ser78 – Ile211
• Endotoxin: <0.2 eu/μg, determined by lal>
• Purity: >95% as analyzed by SDS-PAGE and HPLC.
• Storage: Lyophilized recombinant protein remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhG-DNF should be stable up to 1 week at 4°C or up to 2 months at -20°C.
• Reconstitution: Reconstituted in ddH2O or PBS at 100 μg/ml.

Gene Information

• Gene Name: GDNF glial cell derived neurotrophic factor [ Homo sapiens ]
• Official Symbol: GDNF
• Synonyms: GDNF; glial cell derived neurotrophic factor; glial cell line-derived neurotrophic factor; astrocyte derived trophic factor; ATF1; ATF2; glial cell line derived neurotrophic factor; glial derived neurotrophic factor; HFB1 GDNF; ATF; astrocyte-derived trophic factor; HSCR3; HFB1-GDNF
• Gene ID: 2668
• mRNA Refseq: NM_000514
• Protein Refseq: NP_000505
• MIM: 600837
• UniProt ID: P39905
• Chromosome Location: 5p13.1-p12
• Pathway: Axon guidance, organism-specific biosystem; Developmental Biology, organism-specific biosystem; NCAM signaling for neurite out-growth, organism-specific biosystem; NCAM1 interactions, organism-specific biosystem; Signaling events regulated by Ret tyrosine kinase, organism-specific biosystem
• Function: growth factor activity; protein homodimerization activity; receptor binding