Recombinant Human PDGFB protein
Cat.No. : | PDGFB-528H |
Product Overview : | Recombinant Human PDGFB protein was expressed in Escherichia coli. |
- Specification
- Gene Information
- Related Products
- Download
Description : | Platelet-derived growth factor (PDGF) presenting in serum but absent from plasma was first discovered in animal study by Lynch and co-workers in the late 1980s. It is a disulfide-linked dimer consisting of two peptides-chain A and chain B. PDGF has three subforms: PDGF-AA, PDGF-BB, PDGF-AB. It is involved in a number of biological processes, including hyperplasia, embryonic neuron development, chemotaxis, and respiratory tubule epithelial cell development. The function of PDGF is mediated by two receptors (PDGFR-α and PDGFR-β). |
Source : | E.coli |
Species : | Human |
Form : | Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH 7.4. |
Bio-activity : | Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 3 ng/ml, corresponding to a specific activity of > 3.3 × 10⁵ IU/mg. |
Molecular Mass : | Approximately 24.8 kDa, a disulfide-linked homodimeric protein containing two 110 amino acid residues polypeptide (B chain). |
Protein length : | 110 |
AA Sequence : | MSLGSLTIAEPAMIAECKTRTEVFE ISRRLIDRTNANFLVWPPCVEVQRC SGCCNNRNVQCRPTQVQLRPVQVRK IEIVRKKPIFKKATVTLEDHLACKC ETVAAARPVT |
Endotoxin : | Less than 1 EU/μg of rHuPDGF-BB as determined by LAL method. |
Purity : | >97% by SDS-PAGE and HPLC analysis. |
Storage : | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution. |
Reconstitution : | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤-20 centigrade. Further dilutions should be made in appropriate buffered solutions. |
Gene Name : | PDGFB |
Official Symbol : | PDGFB |
Synonyms : | PDGFB; platelet-derived growth factor beta polypeptide; platelet derived growth factor beta polypeptide (simian sarcoma viral (v sis) oncogene homolog) , SIS; platelet-derived growth factor subunit B; becaplermin; oncogene SIS; SSV; PDGF-2; PDGF, B chain; PDGF subunit B; proto-oncogene c-Sis; platelet-derived growth factor 2; platelet-derived growth factor B chain; platelet-derived growth factor, B chain; Platelet-derived growth factor, beta polypeptide (oncogene SIS); platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog); SIS; PDGF2; c-sis; FLJ12858; |
Gene ID : | 5155 |
mRNA Refseq : | NM_002608 |
Protein Refseq : | NP_002599 |
MIM : | 190040 |
UniProt ID : | P01127 |
Products Types
◆ Recombinant Protein | ||
PDGFB-4406C | Recombinant Chicken PDGFB Protein | +Inquiry |
PDGFB-6595M | Recombinant Mouse PDGFB Protein, His (Fc)-Avi-tagged | +Inquiry |
PDGFB-1175P | Recombinant Pig PDGFB Protein, His-tagged | +Inquiry |
PDGFB-4410E | Recombinant Equine PDGFB Protein | +Inquiry |
PDGFB-153P | Active Recombinant Human PDGFBB Protein (218 aa) | +Inquiry |
◆ Lysates | ||
PDGFB-1321HCL | Recombinant Human PDGFB cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Inquiry
- Q&As
- Reviews
Q&As (7)
Ask a questionBy potently stimulating the migratory and proliferative capabilities of smooth muscle cells and pericytes, PDGFB plays a pivotal role in regulating angiogenesis and orchestrating vascular remodeling events, thereby ensuring proper tissue perfusion and function.
PDGFB signaling assumes a critical role in fueling tumor growth, angiogenesis, and metastasis. PDGFB, acting through its activation of PDGFRβ, elicits a pro-tumorigenic cascade that promotes tumor cell proliferation, survival, migration, and invasion, while concurrently fueling tumor vascularization and distant metastatic spread.
PDGFB emerges as a significant contributor to the pathogenesis of fibrotic diseases by prompting fibroblast activation and proliferation. These dysregulated processes lead to excessive extracellular matrix deposition and subsequent tissue scarring. Furthermore, PDGFB exacerbates fibrosis through induction of myofibroblast differentiation.
Promisingly, preclinical and clinical studies have investigated diverse approaches targeting the PDGFB/PDGFRβ signaling axis for therapeutic intervention in fibrotic disorders and certain neoplastic conditions. Inhibitors directed against PDGFB or PDGFRβ have exhibited considerable potential, effectively blocking downstream signaling cascades activated by PDGFB, thereby hampering cell proliferation and angiogenesis.
PDGFB, released by platelets and macrophages at inflammatory sites, acts as a key modulator in recruiting and activating fibroblasts. This activation not only promotes collagen synthesis but also initiates tissue remodeling, thus expediting the wound healing cascade and tissue integrity restoration.
Several therapeutic strategies aim to target PDGFB for disease treatment. Inhibitors of PDGFB, such as tyrosine kinase inhibitors, have been developed and used in clinical settings. These inhibitors can selectively block the activation of PDGF receptors and inhibit downstream signaling pathways, thereby suppressing excessive cell proliferation and migration. Additionally, approaches targeting PDGFB expression, such as gene therapy or RNA interference, hold promise as potential treatments for diseases associated with PDGFB dysregulation.
PDGFB orchestrates cellular proliferative responses and drives growth by virtue of its ability to activate PDGFRβ, which in turn triggers downstream signaling cascades facilitating DNA synthesis and regulating critical cell cycle checkpoints.
Customer Reviews (3)
Write a reviewThe seal of the reagent tubes is excellent, preventing evaporation and leakage.
With its unique design and construction, the experimental reagent provides me with a fresh experimental experience.
The stability of this reagent is exceptional, ensuring the reliability of the experiment.
Ask a Question for All PDGFB Products
Required fields are marked with *
My Review for All PDGFB Products
Required fields are marked with *
Inquiry Basket