Recombinant Human WWP1, GST-tagged

• Cat.No.: WWP1-75H
• Product Overview: Recombinant full-length human WWP1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Description: WWP1 or WW domain containing E3 ubiquitin protein ligase 1 belongs to a family of NEDD4-like proteins, which are E3 ubiquitin-ligase molecules and regulate key trafficking decisions, including targeting of proteins to proteosomes or lysosomes. WWP1 are found in all eukaryotes and play an important role in the regulation of a wide variety of cellular functions such as protein degradation, transcription, and RNA splicing. WWP1 negatively regulates TGF-beta signaling by directing the degradation of active components of the TGF-beta network. Wwp1 and Ubc18 function to ubiquitinate substrates that regulate longevity induced by diet restriction.
• Source: Sf9 insect cells using baculovirus
• Species: Human
• Tag: GST
• Form: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Molecular Mass: ~160 kDa
• Purity: >70% by densitometry.
• Applications: Western Blot
• Storage: Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.05 μg/μl

Gene Information

• Gene Name: WWP1 WW domain containing E3 ubiquitin protein ligase 1 [ Homo sapiens ]
• Official Symbol: WWP1
• Synonyms: WWP1; WW domain containing E3 ubiquitin protein ligase 1; NEDD4-like E3 ubiquitin-protein ligase WWP1; AIP5; DKFZP434D2111; WW domain-containing protein 1; atrophin-1 interacting protein 5; atrophin-1-interacting protein 5; TGIF-interacting ubiquitin ligase 1; Nedd-4-like ubiquitin-protein ligase; Tiul1; hSDRP1; DKFZp434D2111
• Gene ID: 11059
• mRNA Refseq: NM_007013
• Protein Refseq: NP_008944
• MIM: 602307
• UniProt ID: Q9H0M0
• Chromosome Location: 8q21
• Pathway: Adaptive Immune System, organism-specific biosystem; Antigen processing: Ubiquitination and Proteasome degradation, organism-specific biosystem; Class I MHC mediated antigen processing & presentation, organism-specific biosystem; Downregulation of ERBB4 signaling, organism-specific biosystem; Endocytosis, organism-specific biosystem
• Function: acid-amino acid ligase activity; ligase activity; protein binding; ubiquitin-protein ligase activity; ubiquitin-protein ligase activity

Reviews

08/14/2022

The quality of WWP1 is stable and we trust them.

05/02/2022

Catalytic activity of WWP1 is so powerful that it can easily realize chemical reactions that would otherwise be difficult to perform, and its application prospects are very bright.

07/27/2021

I appreciate the pursuit of product quality by the producers of WWP1, and their product is very reproducible, which makes me more confident in the experimental results.

Q&As

How to use WWP1 protein for disease diagnosis?

The onset, progression, and prognosis of the disease can be assessed by examining the expression level, activity, and interaction with other proteins of the WWP1 protein. These features can also be used to develop new biomarkers and drug targets.

What is the relationship between WWP1 protein and the onset and progression of disease?

Some studies have shown that abnormal expression of WWP1 protein may be related to the occurrence and progression of some diseases. For example, in some cancers, the expression level of the WWP1 protein may change. In addition, the WWP1 protein may also be a drug target for the treatment of these diseases.

How does WWP1 protein relate to other apoptotic pathways?

WWP1 protein has some correlation with other apoptotic pathways. For example, it may be interrelated with apoptotic proteases such as CASP3 to jointly regulate the apoptosis process in cells. In addition, it may be associated with other apoptotic pathways, such as the mitochondrial pathway and the death receptor pathway.

What diseases are associated with mutations or abnormal expression of the WWP1 protein?

Some studies have shown that mutations or abnormal expression of the WWP1 protein may be related to some diseases, such as cancer, autoimmune diseases, etc. However, the specific association needs to be confirmed by further research.

Is WWP1 protein similar to other proteins?

It has certain similarities with other proteins, such as they are involved in the regulation of apoptosis. However, the structure and function of the WWP1 protein are somewhat different from other proteins.

How to use the activity of WWP1 protein for disease treatment?

The activity can be modulated by drugs or other small molecule compounds to treat diseases related to WWP1 protein. For example, some drugs can inhibit the activity of the WWP1 protein, thereby promoting apoptosis and clearing abnormal cells.