Recombinant Human GUCY2C 293 Cell Lysate

• Cat.No.: GUCY2C-5675HCL

Specification

• Description: Antigen standard for guanylate cyclase 2C (heat stable enterotoxin receptor) (GUCY2C) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid (True ORF cDNA clone RC213901, OriGene Technologies, Inc.) and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: E, IP, WB. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: GUCY2C guanylate cyclase 2C (heat stable enterotoxin receptor) [ Homo sapiens ]
• Official Symbol: GUCY2C
• Synonyms: GUCY2C; guanylate cyclase 2C (heat stable enterotoxin receptor); GUC2C; heat-stable enterotoxin receptor; STAR; GC-C; hSTAR; STA receptor; guanylyl cyclase C; intestinal guanylate cyclase
• Gene ID: 2984
• mRNA Refseq: NM_004963
• Protein Refseq: NP_004954
• MIM: 601330
• UniProt ID: P25092
• Chromosome Location: 12p12
• Pathway: Purine metabolism, organism-specific biosystem; Purine metabolism, conserved biosystem
• Function: ATP binding; GTP binding; guanylate cyclase activity; nucleotide binding; protein binding; protein kinase activity; receptor activity; toxin binding; transferase activity, transferring phosphorus-containing groups