Active Recombinant Human CST5, His-tagged

• Cat.No.: CST5-494H
• Product Overview: Recombinant Human CST5 (Accession # CAA49838) Thr29-Val142, fused with a C-terminal 10-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Human
• Tag: His
• Predicted N Terminal: Thr29
• Form: Lyophilized from a 0.2 µm filtered solution in MES and NaCl.
• Bio-activity: Measured by its ability to inhibit papain cleavage of a fluorogenic peptide substrate Z-FR-AMC .The IC50 value is <8 nM, under the described conditions.
• Molecular Mass: Recombinant Human CST5, His-tagged has a calculated MW of 15 kDa. In SDS-PAGE migrates as 15 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: CST5 cystatin D [ Homo sapiens ]
• Official Symbol: CST5
• Synonyms: CST5; cystatin D; cystatin-D; cystatin 5; cystatin-5; cysteine-proteinase inhibitor; MGC71922
• Gene ID: 1473
• mRNA Refseq: NM_001900
• Protein Refseq: NP_001891
• MIM: 123858
• UniProt ID: P28325
• Chromosome Location: 20p11.21