|Cat. No. :
|Product Overview :
||Recombinant Humanhypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) produced inE.Coliis a single, non-glycosylated polypeptide chain. The protein contains 298(530-826) amino acids and has a molecular mass of 32.8 kDa. The protein migrates as a 40 kDa band on SDS-PAGE. The HIF1-A is purified by proprietary chromatographic techniques.
||Hypoxia-inducible factor-1 (HIF-1), identified as one of the transcription factors, has been found to play an essential role in oxygen homeostasis. HIF-1 is a heterodimer composed of HIF-1β subunit and one of three subunits(Hif-1α, Hif-2( or Hif-3)). The activation of Hif-1( is closely associated with a variety of tumors and oncogenic pathways. Hif-1( consists of DNA binding domain(DBD domain), Dimerization domain and C-terminla regulatiory domains, including two transactivation domains(TAD), an oxygen-dependent degradation(ODD) domain, and inhibitory domains.
|Amino Acid Sequence :
||MEFKLELVEK LFAEDTEAKN PFSTQDTDLD LEMLAPYIPM DDDFQLRSFD QLSPLESSSA SPESASPQST VTVFQQTQIQ EPTANATTTT ATTDELKTVT KDRMEDIKIL IASPSPTHIH KETTSATSSP YRDTQSRTAS PNRAGKGVIE QTEKSHPRSP NVLSVALSQR TTVPEEELNP KILALQNAQR KRKMEHDGSL FQAVGIGTLL QQPDDHAATT SLSWKRVKGC KSSEQNGMEQ KTIILIPSDL ACRLLGQSMD ESGLPQLTSY DCEVNAPIQG SRNLLQGEEL LRALDQVN.
|Physical Appearance :
||Sterile filtered liquid formulation 1mg/ml.
||The HIF1A recombinant Human solution is formulated 20mM Tris pH-7.5 and 1mM DTT.
||Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
||Recombinant Human MOP1 although stable at 4°C for 30 days, should be stored desiccated below -20°C for periods greater than 30 days. Please avoid freeze-thaw cycles.