Recombinant Human HIF1A Protein, T7-His-TEV-tagged

• Cat.No.: HIF1A-22H
• Product Overview: Recombinant full-length human HIF1a cDNA (825aa) was constructed with codon optimization using gene synthesis technology and expressed with a small T7-His-TEV cleavage site Tag (29aa) fusion at its N-terminal in E. coli as inclusion bodies.

Specification

• Description: This gene encodes the alpha subunit of transcription factor hypoxia-inducible factor-1 (HIF-1), which is a heterodimer composed of an alpha and a beta subunit. HIF-1 functions as a master regulator of cellular and systemic homeostatic response to hypoxia by activating transcription of many genes, including those involved in energy metabolism, angiogenesis, apoptosis, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF-1 thus plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene.
• Source: E. coli
• Species: Human
• Tag: T7-His-TEV
• Form: Liquid
• AA Sequence: MASMTGGQQMGRGHHHHHHENLYFQ GGEFGSEGAGGANDKKKISSERRKE KSRDAARSRRSKESEVFYELAHQLP LPHNVSSHLDKASVMRLTISYLRVR KLLDAGDLDIEDDMKAQMNCFYLKA LDGFVMVLTDDGDMIYISDNVNKYM GLTQFELTGHSVFDFTHPCDHEEMR EMLTHRNGLVKKGKEQNTQRSFFLR MKCTLTSRGRTMNIKSATWKVLHCT GHIHVYDTNSNQPQCGYKKPPMTCL VLICEPIPHPSNIEIPLDSKTFLSR HSLDMKFSYCDERITELMGYEPEEL LGRSIYEYYHALDSDHLTKTHHDMF TKGQVTTGQYRMLAKRGGYVWVETQ ATVIYNTKNSQPQCIVCVNYVVSGI IQHDLIFSLQQTECVLKPVESSDMK MTQLFTKVESEDTSSLFDKLKKEPD ALTLLAPAAGDTIISLDFGSNDTET DDQQLEEVPLYNDVMLPSPNEKLQN INLAMSPLPTAETPKPLRSSADPAL NQEVALKLEPNPESLELSFTMPQIQ DQTPSPSDGSTRQSSPEPNSPSEYC FYVDSDMVNEFKLELVEKLFAEDTE AKNPFSTQDTDLDLEMLAPYIPMDD DFQLRSFDQLSPLESSSASPESASP QSTVTVFQQTQIQEPTANATTTTAT TDELKTVTKDRMEDIKILIASPSPT HIHKETTSATSSPYRDTQSRTASPN RAGKGVIEQTEKSHPRSPNVLSVAL SQRTTVPEEELNPKILALQNAQRKR KMEHDGSLFQAVGIGTLLQQPDDHA ATTSLSWKRVKGCKSSEQNGMEQKT IILIPSDLACRLLGQSMDESGLPQL TSYDCEVNAPIQGSRNLLQGEELLR ALDQVN
• Purity: > 90% by SDS-PAGE.
• Applications: 1. May be used for in vitro HIF1a mediated gene transcription regulation study in homeostatic response to hypoxia for various cancer cells by intracellular delivery of this protein with protein delivery reagent such as ProFectin reagent kit.
  2. May be used for mapping protein-protein interaction.
  3. May be used as specific substrate protein for kinase, and ubiquitin (Sumo pathway) related enzyme functional screening assays.
  4. Potential biomarker protein / therapeutic target protein for cancer prognosis and cancer treatment drug development.
  5. As immunogen for specific antibody production.
• Stability: Product is stable at 4 centigrade for at least 30 days.
• Storage: Keep at -80 centigrade for long term storage.
• Storage Buffer: 0.2 mg/ml, sterile-filtered, in 20 mM pH 8.0 Tris-HCl Buffer, with proprietary formulation of NaCl, KCl, EDTA, Sucrose and DTT.

Gene Information

• Gene Name: HIF1A
• Official Symbol: HIF1A hypoxia inducible factor 1 subunit alpha [ Homo sapiens (human) ]
• Synonyms: HIF1A; hypoxia inducible factor 1 subunit alpha; HIF1; MOP1; PASD8; HIF-1A; bHLHe78; HIF-1alpha; HIF1-ALPHA; HIF-1-alpha; hypoxia-inducible factor 1-alpha; ARNT interacting protein; PAS domain-containing protein 8; basic-helix-loop-helix-PAS protein MOP1; class E basic helix-loop-helix protein 78; hypoxia inducible factor 1 alpha subunit; hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor); hypoxia-inducible factor 1 alpha isoform I.3; hypoxia-inducible factor1alpha; member of PAS protein 1; member of PAS superfamily 1
• Gene ID: 3091
• mRNA Refseq: NM_001530
• Protein Refseq: NP_001521
• MIM: 603348
• UniProt ID: Q16665