Recombinant Human NQO1, His-tagged

• Cat.No.: NQO1-29158TH
• Product Overview: Recombinant full length Human NQO1 fused to His tag at N-terminal; amino acids 1-274 , 33kDa.

Specification

• Description: This gene is a member of the NAD(P)H dehydrogenase (quinone) family and encodes a cytoplasmic 2-electron reductase. This FAD-binding protein forms homodimers and reduces quinones to hydroquinones. This proteins enzymatic activity prevents the one electron reduction of quinones that results in the production of radical species. Mutations in this gene have been associated with tardive dyskinesia (TD), an increased risk of hematotoxicity after exposure to benzene, and susceptibility to various forms of cancer. Altered expression of this protein has been seen in many tumors and is also associated with Alzheimers disease (AD). Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
• Conjugation: HIS
• Source: E. coli
• Form: Liquid
• Purity: >95% by SDS-PAGE
• Storage buffer: Preservative: NoneConstituents: 10% Glycerol, 20mM Tris HCl, 1mM DTT, pH 8
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Sequences of amino acids: AMKEAAAAAL KKKGWEVVES DLYAMNFNPI ISRKDITGKL KDPANFQYPA ESVLAYKEGH LSPDIVAEQK KLEAADLVIF QFPLQWFGVP AILKGWFERV FIGEFAYTYA AMYDKGPFRS KKAVLSITTG GSGSMYSLQG IHGDMNVILW PIQSGILHFC GFQVLEPQLT YSIGHTPADA RIQILEGWKK RLENIWDETP LYFAPSSLFD LNFQAGFLMK KEVQDEEKNK KFGLSVGHHL GKSIPTDNQI KARK
• Sequence Similarities: Belongs to the NAD(P)H dehydrogenase (quinone) family.

Gene Information

• Gene Name: NQO1 NAD(P)H dehydrogenase, quinone 1 [ Homo sapiens ]
• Official Symbol: NQO1
• Synonyms: NQO1; NAD(P)H dehydrogenase, quinone 1; DIA4, diaphorase (NADH/NADPH) (cytochrome b 5 reductase) , NMOR1; NAD(P)H dehydrogenase [quinone] 1; DHQU; DTD; QR1
• Gene ID: 1728
• mRNA Refseq: NM_000903
• Protein Refseq: NP_000894
• Uniprot ID: P15559
• Chromosome Location: 16q12-q22
• Pathway: Keap1-Nrf2 Pathway, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of amino acids and derivatives, organism-specific biosystem; Oxidative Stress, organism-specific biosystem; Regulation of ornithine decarboxylase (ODC), organism-specific biosystem
• Function: NAD(P)H dehydrogenase (quinone) activity; coenzyme binding; cytochrome-b5 reductase activity; electron carrier activity; oxidoreductase activity

Q&As

How are mutations in NQO1 detected and analyzed?

Mutations in NQO1 can be detected and analyzed by methods such as whole-genome sequencing or target region sequencing to understand the impact of mutations on protein structure and function.

What are the mutation types of NQO1?

The types of mutations in NQO1 include point mutations, insertions/deletions, duplications, etc., which can cause structural and functional abnormalities of the protein.

Are abnormal NQO1 levels indicative of a disease?

Abnormal NQO1 levels may indicate cancer, neurodegenerative diseases, and oxidative stress-related diseases.

How to study the regulatory mechanism of NQO1?

Studying the regulatory mechanism of NQO1 requires a combination of various experimental methods and techniques, such as gene knockout, transcriptome analysis, and protein-protein interactions.

What are the applications of NQO1 in medical treatment?

This protein can be used as a biomarker in medical treatment to help monitor disease progression and treatment efficacy. In addition, therapeutics targeting NQO1, such as gene therapy or modulating its expression, are also being studied.

Is there a relationship between NQO1 and other genes or proteins?

NQO1 has a complex relationship with other genes or proteins, and can interact with other genes or proteins and participate in a variety of biochemical reactions.