Recombinant Human Human PDGFA

• Cat.No.: PDGFB-31597TH
• Product Overview: Recombinant Human PDGFA + PDGFB is a heterodimeric, non-glycosylated, polypeptide chain containing 234 amino acids consisting of a 13.3kDa and 12.2 kDa ,having a total molecular mass of 25.5kDa.100%identity with P04085 over 87-211 aa positions of the pr

Specification

• Description: The protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma. Reciprocal translocations between chromosomes 22 and 7, at sites where this gene and that for COL1A1 are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans resulting from unregulated expression of growth factor. Two alternatively spliced transcript variants encoding different isoforms have been identified for this gene.
• Source: E. coli
• Biological activity: Specific Activity of 1 MIU/mg.
• Form: Lyophilised:It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.
• Purity: >95% by SDS-PAGE
• Storage buffer: Preservative: NoneConstituents: 10mM Acetic acid
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Sequences of amino acids: alpha chain:-MSIEEAVPAV CKTRTVIYEI PRSQVDPTSA NFLIWPPCVE VKRCTGCCNT SSVKCQPSRV HHRSVKVAKV EYVRKKPKLK EVQVRLEEHL ECACATTSLN PDYREEDTGR PRESGKKRKR KRLKPTbeta chain:-MSLGSLTIAE PAMIAECKTR TEVFEISRRL IDRTNANFLV WPPCVEVQRC SGCCNNRNVQ CRPTQVQLRP VQVRKIGIVR KKPIFKKATV TLGDHLACKC ETVAAARPVT

Gene Information

• Gene Name: PDGFB platelet-derived growth factor beta polypeptide [ Homo sapiens ]
• Official Symbol: PDGFB
• Synonyms: PDGFB; platelet-derived growth factor beta polypeptide; platelet derived growth factor beta polypeptide (simian sarcoma viral (v sis) oncogene homolog) , SIS; platelet-derived growth factor subunit B; becaplermin; oncogene SIS; SSV
• Gene ID: 5155
• mRNA Refseq: NM_002608
• Protein Refseq: NP_002599
• MIM: 190040
• Uniprot ID: P01127
• Chromosome Location: 22q13.1
• Pathway: Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Downstream signal transduction, organism-specific biosystem; Focal Adhesion, organism-specific biosystem; Focal adhesion, organism-specific biosystem
• Function: cell surface binding; collagen binding; eukaryotic cell surface binding; growth factor activity; platelet-derived growth factor binding

Reviews

12/27/2018

The seal of the reagent tubes is excellent, preventing evaporation and leakage.

05/08/2018

With its unique design and construction, the experimental reagent provides me with a fresh experimental experience.

03/21/2016

The stability of this reagent is exceptional, ensuring the reliability of the experiment.

Q&As

In what capacity does PDGFB contribute to the intricate processes of angiogenesis and vascular remodeling?

By potently stimulating the migratory and proliferative capabilities of smooth muscle cells and pericytes, PDGFB plays a pivotal role in regulating angiogenesis and orchestrating vascular remodeling events, thereby ensuring proper tissue perfusion and function.

How does PDGFB intricately contribute to the development and advancement of certain malignancies?

PDGFB signaling assumes a critical role in fueling tumor growth, angiogenesis, and metastasis. PDGFB, acting through its activation of PDGFRβ, elicits a pro-tumorigenic cascade that promotes tumor cell proliferation, survival, migration, and invasion, while concurrently fueling tumor vascularization and distant metastatic spread.

In what ways is PDGFB implicated in the pathogenesis and progression of fibrotic disorders?

PDGFB emerges as a significant contributor to the pathogenesis of fibrotic diseases by prompting fibroblast activation and proliferation. These dysregulated processes lead to excessive extracellular matrix deposition and subsequent tissue scarring. Furthermore, PDGFB exacerbates fibrosis through induction of myofibroblast differentiation.

What novel therapeutic avenues are being explored targeting the PDGFB/PDGFRβ signaling axis for interventional strategies?

Promisingly, preclinical and clinical studies have investigated diverse approaches targeting the PDGFB/PDGFRβ signaling axis for therapeutic intervention in fibrotic disorders and certain neoplastic conditions. Inhibitors directed against PDGFB or PDGFRβ have exhibited considerable potential, effectively blocking downstream signaling cascades activated by PDGFB, thereby hampering cell proliferation and angiogenesis.

How does PDGFB actively participate in the intricate mechanisms governing wound healing and tissue regeneration?

PDGFB, released by platelets and macrophages at inflammatory sites, acts as a key modulator in recruiting and activating fibroblasts. This activation not only promotes collagen synthesis but also initiates tissue remodeling, thus expediting the wound healing cascade and tissue integrity restoration.

What therapeutic strategies target PDGFB for disease treatment?

Several therapeutic strategies aim to target PDGFB for disease treatment. Inhibitors of PDGFB, such as tyrosine kinase inhibitors, have been developed and used in clinical settings. These inhibitors can selectively block the activation of PDGF receptors and inhibit downstream signaling pathways, thereby suppressing excessive cell proliferation and migration. Additionally, approaches targeting PDGFB expression, such as gene therapy or RNA interference, hold promise as potential treatments for diseases associated with PDGFB dysregulation.

How does PDGFB orchestrate cellular proliferation and growth in diverse tissues and organs?

PDGFB orchestrates cellular proliferative responses and drives growth by virtue of its ability to activate PDGFRβ, which in turn triggers downstream signaling cascades facilitating DNA synthesis and regulating critical cell cycle checkpoints.