Recombinant Human DNMT3A, GST-tagged

• Cat.No.: DNMT3A-513H
• Product Overview: Recombinant Human DNMT3a, (a-a 2-913), with N-terminal GST tag expressed inbaculovirus. MW= 128 kDa.

Specification

• Cat. No.: DNMT3A-513H
• Description: CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated. Alternative splicing results in multiple transcript variants encoding different isoforms.
• Source: Baculovirus.
• Purity: >50%.
• Application: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
• Formulated In: 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20 and 10% glycerol.
• Stability: >6 months at –80°C.

Gene Information

• Gene Name: DNMT3A DNA (cytosine-5-)-methyltransferase 3 alpha [ Homo sapiens ]
• Synonyms: DNMT3A; DNA (cytosine-5-)-methyltransferase 3 alpha; DNMT3A2; M.HsaIIIA; EC 2.1.1.37; DNA MTase HsaIIIA; DNA methyltransferase HsaIIIA; DNA cytosine methyltransferase 3A2; DNA (cytosine-5)-methyltransferase 3A; Dnmt3a; DNA MTase HsaIIIA
• Gene ID: 1788
• mRNA Refseq: NM_022552
• Protein Refseq: NP_072046
• MIM: 602769
• UniProt ID: Q9Y6K1
• Chromosome Location: 2p23
• Pathway: Cysteine and methionine metabolism; Metabolic pathways
• Function: DNA (cytosine-5-)-methyltransferase activity; DNA binding; metal ion binding; chromatin binding; transferase activity; zinc ion binding