Recombinant Human SIRT4

• Cat.No.: SIRT4-30846TH
• Product Overview: Recombinant full length Human SIRT4 with an N terminal proprietary tag; predicted MWt 62 kDa inclusive of tag

Specification

• Description: This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class IV of the sirtuin family.
• Protein length: 314 amino acids
• Molecular Weight: 62.000kDa inclusive of tags
• Source: E. coli
• Tissue specificity: Detected in vascular smooth muscle and striated muscle. Detected in insulin-producing beta-cells in pancreas islets of Langerhans (at protein level). Widely expressed. Weakly expressed in leukocytes and fetal thymus.
• Form: Liquid
• Purity: by SDS-PAGE
• Storage buffer: Preservative: NoneConstituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 10mM Glutathione, 0.25mM DTT, 0.1mM EDTA, 0.1mM PMSF, pH 7.5
• Storage: Shipped on dry ice. Upon delivery aliquot and store at -80oC. Avoid freeze / thaw cycles.
• Sequences of amino acids: MKMSFALTFRSAKGRWIANPSQPCS KASIGLFVPASPPLDPEKVKELQRF ITLSKRLLVMTGAGISTESGIPDYR SEKVGLYARTDRRPIQHGDFVRSAP IRQRYWARNFVGWPQFSSHQPNPAH WALSTWEKLGKLYWLVTQNVDALHT KAGSRRLTELHGCMDRVLCLDCGEQ TPRGVLQERFQVLNPTWSAEAHGLA PDGDVFLSEEQVRSFQVPTCVQCGG HLKPDVVFFGDTVNPDKVDFVHKRV KEADSLLVVGSSLQVYSGYRFILTA WEKKLPIAILNIGPTRSDDLACLKL NSRCGELLPLIDPC
• Sequence Similarities: Belongs to the sirtuin family.Contains 1 deacetylase sirtuin-type domain.

Gene Information

• Gene Name: SIRT4 sirtuin 4 [ Homo sapiens ]
• Official Symbol: SIRT4
• Synonyms: SIRT4; sirtuin 4; sirtuin (silent mating type information regulation 2 homolog) 4 (S. cerevisiae) , sirtuin (silent mating type information regulation 2, S. cerevisiae, homolog) 4; NAD-dependent ADP-ribosyltransferase sirtuin-4; SIR2L4
• Gene ID: 23409
• mRNA Refseq: NM_012240
• Protein Refseq: NP_036372
• MIM: 604482
• Uniprot ID: Q9Y6E7
• Chromosome Location: 12q24.31
• Pathway: Signaling events mediated by HDAC Class I, organism-specific biosystem
• Function: NAD+ ADP-ribosyltransferase activity; NAD+ ADP-ribosyltransferase activity; NAD+ binding; NOT NAD-dependent protein deacetylase activity; metal ion binding