Recombinant Human ECHS1, His-tagged ECHS1-2811H

Cat. No.:	ECHS1-2811H
Product Overview:	Recombinant human ECHS1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. MW = 30.6 kDa (284aa), confirmed by MALDI-TOF.
Description:	Enoyl Coenzyme A hydratase, short chain 1 mitochondrial, also known as ECHS1, is a member of the hydratase/isomerase superfamily. It localizes to the mitochondrial matrix. Expressed in muscle, liver and fibroblasts, with low expression in kidney and spleen, ECHS1 exists as a homohexamer that functions in the second step of the mitochondrial fatty acid β-oxidation pathway.
Source:	E.coli.
Sequences Of Amino Acids:	MGSSHHHHHH SSGLVPRGSH MASGANFEYI IAEKRGKNNT VGLIQLNRPK ALNALCDGLI DELNQALKIF EEDPAVGAIV LTGGDKAFAA GADIKEMQNL SFQDCYSSKF LKHWDHLTQV KKPVIAAVNG YAFGGGCELA MMCDIIYAGE KAQFAQPEIL IGTIPGAGGT QRLTRAVGKS LAMEMVLTGD RISAQDAKQA GLVSKICPVE TLVEEAIQCA EKIASNSKIV VAMAKESVNA AFEMTLTEGS KLEKKLFYST FATDDRKEGM TAFVEKRKAN FKDQ
Purity:	> 95% by SDS – PAGE.
Form:	Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 20% glycerol, 100mM NaCl.
Concentration:	1 mg/ml (determined by Bradford assay).
Storage:	Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

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Gene Name:	ECHS1 enoyl CoA hydratase, short chain, 1, mitochondrial [ Homo sapiens ]
Synonyms:	ECHS1; enoyl CoA hydratase, short chain, 1, mitochondrial; SCEH; mitochondrial short-chain enoyl-coenzyme A hydratase 1; EC 4.2.1.17;OTTHUMP00000020811; Enoyl-CoA hydratase 1; Short chain enoyl-CoA hydratase
Gene ID:	1892
mRNA Refseq:	NM_004092
Protein Refseq:	NP_004083
MIM:	602292
UniProt ID:	P30084
Chromosome Location:	10q26.2-q26.3
Pathway:	Benzoate degradation via CoA ligation; Butanoate metabolism; Caprolactam degradation; Fatty acid elongation in mitochondria; Fatty acid metabolism; Limonene and pinene degradation; Lysine degradation; Propanoate metabolism; Tryptophan metabolism; Valine, leucine and isoleucine degradation ; beta-Alanine metabolism;Lipid and lipoprotein metabolism; Metabolism of amino acids
Function:	enoyl-CoA hydratase activity; enoyl-CoA hydratase activity; lyase activity; protein binding