Recombinant Mouse Echs1 Protein, Myc/DDK-tagged

• Cat.No.: Echs1-2719M
• Product Overview: Purified recombinant protein of mouse full-length enoyl Coenzyme A hydratase, short chain, 1, mitochondrial (Echs1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Straight-chain enoyl-CoA thioesters from C4 up to at least C16 are processed, although with decreasing catalytic rate. Has high substrate specificity for crotonyl-CoA and moderate specificity for acryloyl-CoA, 3-methylcrotonyl-CoA and methacrylyl-CoA. It is noteworthy that binds tiglyl-CoA, but hydrates only a small amount of this substrate.
• Molecular Mass: 31.6 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Echs1 enoyl Coenzyme A hydratase, short chain, 1, mitochondrial [ Mus musculus (house mouse) ]
• Official Symbol: Echs1
• Synonyms: ECHS1; enoyl Coenzyme A hydratase, short chain, 1, mitochondrial; enoyl-CoA hydratase, mitochondrial; SCEH; enoyl-CoA hydratase 1; short chain enoyl-CoA hydratase; short-chain enoyl-CoA hydratase; C80529
• Gene ID: 93747
• mRNA Refseq: NM_053119
• Protein Refseq: NP_444349
• UniProt ID: Q8BH95