Recombinant Human DNMT3A Protein, GST/StrepII-tagged
Cat.No. : | DNMT3A-01H |
Product Overview : | Human recombinant DNMT3a (residues 214-912, C-term) was expressed in Sf9 insect cells with an N-terminal GST-tag and C-terminal StrepII tag. DNA (cytosine-5)-methyltransferase 3a (DNMT3a) is required for de novo DNA methylation and methylates at both CpG and non-CpG sites. |
- Specification
- Gene Information
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Description : | CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated. |
Source : | Insect cell |
Species : | Human |
Tag : | GST/StrepII |
Molecular Mass : | 107 kDa |
Protein length : | 214-912 |
Purity : | > 80% by SDS-PAGE. |
Usage : | DNMT3a (214-912) displays DNA methyltransferase activity at concentrations of 60 nM and above, 120 min. reactions, 30 centigrade, as TCA-precipitated counts in a scintillation/filter plate assay. Reaction conditions are: 50 mM Tris, pH 8, 5 mM EDTA, 10% glycerol, 10 mM BME, 0.05 mM PMSF with 1.5 μM [3H]-SAM and 10 μg/mL Lambda DNA as substrate. |
Concentration : | 1.62 μg/μL |
Storage Buffer : | 50 mM Tris-HCl pH 8.0, 150 mM NaCl, 10% glycerol, 3 mM DTT, 0.05% Tween 20 |
Gene Name : | DNMT3A DNA methyltransferase 3 alpha [ Homo sapiens (human) ] |
Official Symbol : | DNMT3A |
Synonyms : | DNMT3A; DNA methyltransferase 3 alpha; TBRS; HESJAS; DNMT3A2; M.HsaIIIA; DNA (cytosine-5)-methyltransferase 3A; DNA (cytosine-5-)-methyltransferase 3 alpha; DNA MTase HsaIIIA; DNA cytosine methyltransferase 3A2; EC 2.1.1.37 |
Gene ID : | 1788 |
mRNA Refseq : | NM_175629 |
Protein Refseq : | NP_783328 |
MIM : | 602769 |
UniProt ID : | Q9Y6K1 |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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