Recombinant Human ING1 Protein, MYC/DDK-tagged

• Cat.No.: ING1-2405H
• Product Overview: Recombinant Human ING1 protein, fused to MYC/DDK-tagged at C-terminus, was expressed in HEK293.

Specification

• Description: This gene encodes a tumor suppressor protein that can induce cell growth arrest and apoptosis. The encoded protein is a nuclear protein that physically interacts with the tumor suppressor protein TP53 and is a component of the p53 signaling pathway. Reduced expression and rearrangement of this gene have been detected in various cancers. Multiple alternatively spliced transcript variants encoding distinct isoforms have been reported.
• Source: HEK293
• Species: Human
• Tag: MYC/DDK
• Form: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 31.7 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >50 ug/mL as determined by microplate BCA method

Gene Information

• Gene Name: ING1 inhibitor of growth family, member 1 [ Homo sapiens ]
• Official Symbol: ING1
• Synonyms: p24ING1c; p33; p33ING1; p33ING1b; p47; p47ING1a
• Gene ID: 3621
• mRNA Refseq: NM_005537
• Protein Refseq: NP_005528
• MIM: 601566
• UniProt ID: Q9UK53

Reviews

11/07/2020

 The product’s innovative characteristics enabled us to explore previously uncharted territories within cellular mechanisms.

09/03/2019

 The value derived from the product’s usage made the investment worthwhile, considering the knowledge acquired.

03/05/2018

 Replicating results underscored the reliability and relevance of the product’s effects.

Q&As

How was ING1 identified as a candidate tumor antigen for CRC?

ING1 was identified through a protein microarray screening method using protein microarrays (ProtoArray).

In which other types of cancer were increased levels of anti-ING1 antibodies detected?

Elevated levels of anti-ING1 antibodies were observed not only in CRC but also in esophageal cancer (EC), gastric cancer (GC), breast cancer (BrC), and pancreatic cancer (PC).

Are there specific regions of the ING1 protein associated with CRC?

Antibodies against ING1 amino acids between 239 and 253 were found at significantly higher levels in patients with CRC compared to other cancer types.

What was the result of immunohistochemical staining for ING1 protein in CRC cells?

Immunohistochemical staining revealed higher expression of ING1 protein in CRC cells than in adjacent normal tissues.

How do serum anti-ING1 antibody levels in CRC patients compare to healthy donors (HDs)?

Anti-ING1 antibody levels were significantly higher in CRC patients at all stages compared to HDs.

What is the significance of ING1 in the context of colorectal cancer (CRC)?

ING1 has been identified as a candidate tumor antigen for CRC and shows potential as a biomarker for this cancer type.

What effect does ING1 have on specific promoters in CRC cells?

ING1 was found to augment p53-mediated NOXA promoter activity but attenuate p53-stimulated Bax, p21, and PUMA promoter activities in CRC cell line experiments.