Recombinant Human/Mouse IL23A & IL12B protein(Met1-Pro189 & Met1-Ser335), His-tagged

• Cat.No.: IL23A & IL12B-3393H
• Product Overview: Recombinant Human IL23A (Q9NPF7) (Met1-Pro189) was expressed in HEK293, fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; Recombinant Mouse IL12B (P43432) (Met1-Ser335) was expressed in HEK293 with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the Human IL23A & the Mouse IL12B heterodimer was purified.

Specification

• Source: HEK293
• Species: Human/Mouse
• Tag: C-His
• Protein length: Met1-Pro189 & Met1-Ser335
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant human IL23A & the mouse IL12B comprises 505(181+324) amino acids and has a calculated molecular mass of 57.3 (20.1+37.2) KDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.