Recombinant Rhesus IL23A & IL12B protein(Met1-Ser219 & Met1-Ser328), His-tagged

• Cat.No.: IL23A & IL12B-3394R
• Product Overview: Recombinant rhesus IL12A (NP_001038199.1) (Met1-Ser219) was expressed in HEK293, fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; Recombinant rhesus IL12B (NP_001038190.1) (Met1-Ser328) was expressed with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the rhesus IL12A & the rhesus IL12B heterodimer was purified.

Specification

• Source: HEK293
• Species: Rhesus
• Tag: C-His
• Protein length: Met1-Ser219 & Met1-Ser328
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant rhesus IL12A & the rhesus IL12B comprises 525(208+317) amino acids and has a calculated molecular mass of 60.2(24+36.2) KDa.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.