Recombinant Mouse TGFB1(Leu30-Ser390) & GARP(Ala18-Leu628) Protein, His-Avi-tagged, Biotinylated

• Cat.No.: TGFB1 & GARP-3435M
• Product Overview: Biotinylated Recombinant Mouse TGFB1(Leu30-Ser390) & GARP(Ala18-Leu628) Protein(G3XA59(GARP)&P04202(TGFB1)) is expressed from HEK293 with His tag and Avi tag at the N-Terminus.

Specification

• Source: HEK293
• Species: Mouse
• Tag: N-His-Avi
• Protein length: Leu30-Ser390 & Ala18-Leu628
• Conjugation/Label: Biotin
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Mouse ITGAV&ITGB6, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Mouse GARP&Latent TGF beta 1 Complex, His Tag with the EC50 of 0.12μg/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 66.6 kDa (GARP)&44.2 kDa (Latent TGF beta 1). Due to glycosylation, the protein migrates to 72-76 kDa (GARP)&13 kDa&45-47 kDa (Latent TGF beta 1) based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE
  > 95% as determined by HPLC
• Storage: -20 to -80°C for 12 months as supplied from date of receipt.
  -80°C for 3-6 months after reconstitution.
  2-8°C for 2-7 days after reconstitution.
  Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: Tgfb1 transforming growth factor, beta 1 [ Mus musculus ]
• Official Symbol: TGFB1
• Synonyms: TGFB1; transforming growth factor, beta 1; transforming growth factor beta-1; TGF-beta 1; TGF-beta-1; regulatory protein; transforming growth factor-beta 1; Tgfb; Tgfb-1; TGFbeta1; TGF-beta1
• Gene ID: 21803
• mRNA Refseq: NM_011577
• Protein Refseq: NP_035707