Recombinant Rat TGFB1(Leu30-Arg278) & GARP(Ala18-Leu628) Protein, His-Avi-tagged, Biotinylated
Cat.No. : | TGFB1 & GARP-3437R |
Product Overview : | Biotinylated Recombinant Rat TGFB1(Leu30-Arg278) & GARP(Ala18-Leu628) Protein is expressed from HEK293 with His tag and Avi Tag at the C-Terminus. |
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Source : | HEK293 |
Species : | Rat |
Tag : | C-His-Avi |
Protein length : | Leu30-Arg278 & Ala18-Leu628 |
Conjugation/Label : | Biotin |
Form : | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Bio-activity : | Immobilized Human ITGAV&ITGB6, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Rat GARP&Latent TGF Beta 1 Complex, His Tag with the EC50 of 0.4μg/ml determined by ELISA. |
Molecular Mass : | The protein has a predicted MW of 69.80 kDa (GARP)&41.28 kDa (Latent TGF beta 1). Due to glycosylation, the protein migrates to 70-80 kDa (GARP)&42-48 kDa&13 kDa (Latent TGF beta 1) based on Tris-Bis PAGE result. |
Endotoxin : | Less than 1EU per μg by the LAL method. |
Purity : | > 95% as determined by Tris-Bis PAGE > 95% as determined by HPLC |
Storage : | -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Reconstitution : | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Gene Name : | Tgfb1 transforming growth factor, beta 1 [ Rattus norvegicus ] |
Official Symbol : | TGFB1 |
Synonyms : | TGFB1; transforming growth factor, beta 1; transforming growth factor beta-1; TGF-beta-1; transforming growth factor, beta-1; Tgfb; |
Gene ID : | 59086 |
mRNA Refseq : | NM_021578 |
Protein Refseq : | NP_067589 |
Products Types
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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